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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1976-12-30
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pubmed:abstractText |
Lipolysaccharide was isolated from Chromatium vinosum by phenol/water extraction. The lipopolysaccharide is found exclusively in the phenol phase and can be cleaved into a sugar moiety and a lipid A fraction by hydrolysis in 10% acetic acid at 100 degrees C for 3-4 h. The sugar moiety contains the neutral sugars 3-O-methyl-D-ribose, D-ribose, L-arabinose, mannosamine and glucose, and smaller quantities of D-rhamnose, D-glycero-D-manno-heptose (tentatively identified), quinovosamine and 2-keto-3-deoxyoctonate. L-glycero-D-manno-heptose was not detected. The 2-keto-3-deoxyoctonate linkage in C. vinosum lipopolysaccharide is more resistant to acid hydrolysis than that of Escherichia coli. The lipid A fraction contains glucosamine, mannose and the fatty acids of the lipopolysaccharide. The major fatty acid is beta-hydroxymyristic acid, with smaller amounts of lauric and palmitic acids as well as 14-carbon mono-unsaturated fatty acid, also being present. The phosphorus content of the C. vinosum lipopolysaccharide was found to be approximately 0.1%. Erythrocytes sensitized with alkali-treated C. vinosum lipopolysaccharide were agglutinated by antisera prepared against heat-killed cells. Untreated or heat-treated lipopolysaccharide did not sensitize erythrocytes. The lethal toxicity to mice of the C. vinosum lipopolysaccharide is about one-tenth as that from Salmonella abortus equi.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Carbohydrates,
http://linkedlifedata.com/resource/pubmed/chemical/Fatty Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphates,
http://linkedlifedata.com/resource/pubmed/chemical/Polysaccharides, Bacterial
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0014-2956
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
68
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
365-71
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pubmed:dateRevised |
2007-7-23
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pubmed:meshHeading |
pubmed-meshheading:976262-Animals,
pubmed-meshheading:976262-Biological Assay,
pubmed-meshheading:976262-Carbohydrates,
pubmed-meshheading:976262-Chromatium,
pubmed-meshheading:976262-Dose-Response Relationship, Drug,
pubmed-meshheading:976262-Erythrocytes,
pubmed-meshheading:976262-Fatty Acids,
pubmed-meshheading:976262-Hemagglutination Tests,
pubmed-meshheading:976262-Lipopolysaccharides,
pubmed-meshheading:976262-Mice,
pubmed-meshheading:976262-Phosphates,
pubmed-meshheading:976262-Polysaccharides, Bacterial,
pubmed-meshheading:976262-Rabbits
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pubmed:year |
1976
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pubmed:articleTitle |
Isolation and characterization of the lipopolysaccharide of Chromatium vinosum.
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pubmed:publicationType |
Journal Article
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