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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1998-11-6
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pubmed:abstractText |
Agarose disc gel electrophoresis has been adapted to achieve the separation of the major sulfated glycosaminoglycans produced by cells in culture. By use of buffers containing barium ion, mixtures of chondroitin sulfate, dermatan sulfate, and heparan sulfate are well resolved into discrete bands. The technique can be used preparatively as well as analytically to separate quantities of glycosaminoglycans up to a milligram in a 6-mm diameter gel.
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pubmed:grant |
|
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pubmed:language |
eng
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pubmed:journal |
|
|
pubmed:citationSubset |
IM
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pubmed:chemical |
|
|
pubmed:status |
MEDLINE
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pubmed:month |
Dec
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|
pubmed:issn |
0003-2697
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:volume |
91
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
464-72
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:9762132-Animals,
pubmed-meshheading:9762132-Barium,
pubmed-meshheading:9762132-Cattle,
pubmed-meshheading:9762132-Cells, Cultured,
pubmed-meshheading:9762132-Chondroitin Sulfates,
pubmed-meshheading:9762132-Dermatan Sulfate,
pubmed-meshheading:9762132-Electrophoresis, Agar Gel,
pubmed-meshheading:9762132-Glycosaminoglycans,
pubmed-meshheading:9762132-Heparitin Sulfate
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|
pubmed:year |
1978
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|
pubmed:articleTitle |
An agarose gel electrophoretic method for analysis of sulfated glycosaminoglycans of cultured cells.
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pubmed:affiliation |
Department of Ophthalmology, University of Washington, Seattle 98195, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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