Linked Life Data

9762089

Source:http://linkedlifedata.com/resource/pubmed/id/9762089

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1998-11-6
pubmed:abstractText
An improved method for extraction and purification of soluble elastin from aortas of copper-deficient swine has been devised. It depends upon the use of both acidic and neutral protease inhibitors during preparation. Collagen is first precipitated with acetic acid. A two-step separation and purification of elastin from the collagen-free extract is based on absorption of the acidic proteins on DEAE-cellulose and gel filtration through agarose. The protein recovered is homogeneous by gel electrophoresis. It has the molecular weight (75,000) and amino acid composition of the soluble elastin from the same source prepared by repeated coacervation.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0003-2697
pubmed:author
pubmed:issnType
Print
pubmed:volume
91
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
115-22
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1978
pubmed:articleTitle
Chromatographic purification of soluble elastin.
pubmed:affiliation
Department of Pathology, University of California, School of Medicine, Los Angeles 90024, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.