Linked Life Data

9761462

Source:http://linkedlifedata.com/resource/pubmed/id/9761462

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1998-12-11
pubmed:abstractText
This paper describes Western-blotting evidence for the presence of various guanine nucleotide binding proteins, G-proteins in cultured rat cerebral endothelial cells (CECs) and two immortalized cerebral endothelial cell lines, RBE4 and GP8. By using specific antibodies raised against known sequences of appropriate G-protein types that were previously characterized, we demonstrated the presence of Gsalpha, Gi2alpha, Gi3alpha, Gq/11alpha, Goalpha and Gbeta in cell lysates of primary cultures of CECs, and plasma membranes of RBE4 and GP8 cells. The appearance of Goalpha proteins in CECs might be of special importance, since they were not detected in peripheral endothelial cells in previous studies. Isoproterenol and bradykinin displayed significant, dose-dependent stimulation of [35S]GTPgammaS binding above basal values. This assay, reflecting the GDP-GTP exchange reaction on Galpha-subunits by receptor agonists, suggested that there were functional, G-protein coupled beta-adrenergic and bradykinin receptors in these systems. No significant stimulation of [35S]GTP7gammaS binding was noted with serotonin under our experimental conditions. Since stimulation of [35S]GTPgammaS binding by isoproterenol and bradykinin was additive, it was concluded that different Galpha proteins were activated by these two ligands. In analogy to other systems, activation of Gs is most likely by isoproterenol, while Gi and/or Gq/11 proteins might be activated by bradykinin receptors. The possible significance of the receptors and G-proteins detected is being discussed in the functioning of cerebral endothelium, and thus the blood-brain barrier.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0197-0186
pubmed:author
pubmed:issnType
Print
pubmed:volume
33
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
179-85
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:9761462-Animals, pubmed-meshheading:9761462-Blotting, Western, pubmed-meshheading:9761462-Bradykinin, pubmed-meshheading:9761462-Brain, pubmed-meshheading:9761462-Cell Line, pubmed-meshheading:9761462-Cell Membrane, pubmed-meshheading:9761462-Cells, Cultured, pubmed-meshheading:9761462-Endothelium, Vascular, pubmed-meshheading:9761462-GTP-Binding Protein alpha Subunits, Gi-Go, pubmed-meshheading:9761462-GTP-Binding Protein alpha Subunits, Gs, pubmed-meshheading:9761462-GTP-Binding Proteins, pubmed-meshheading:9761462-Guanosine 5'-O-(3-Thiotriphosphate), pubmed-meshheading:9761462-Immunoblotting, pubmed-meshheading:9761462-Isoproterenol, pubmed-meshheading:9761462-Rats, pubmed-meshheading:9761462-Receptors, Adrenergic, beta, pubmed-meshheading:9761462-Receptors, Bradykinin
pubmed:year
1998
pubmed:articleTitle
Expression of G-protein subtypes in cultured cerebral endothelial cells.
pubmed:affiliation
Institute of Biochemistry, Biological Research Center of the Hungarian Academy of Sciences, Szeged.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't