Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
7
|
| pubmed:dateCreated |
1998-10-22
|
| pubmed:abstractText |
The involvement of granulocyte-macrophage CSF (GM-CSF) in collagen-induced arthritis (CIA) was examined using GM-CSF-deficient mice. Although CIA is generally considered to be restricted to mice of the H-2q or H-2r haplotypes, we examined the role of GM-CSF in the CIA model using GM-CSF-deficient (-/-) and wild-type (+/+) mice on a C57BL/6 (H-2b) background. Mice were immunized by intradermal injection at the base of the tail with chick type II collagen followed by a repeat injection 21 days later. We found, based on both clinical and histologic assessments, that wild-type mice on this background developed severe CIA, while the GM-CSF-deficient mice had virtually no disease. Mice that were heterozygous for the GM-CSF gene (+/-) collectively displayed an intermediate response between those of the GM-CSF(+/+) and GM-CSF(-/-) groups, suggesting a gene dosage effect. GM-CSF(+/+) and GM-CSF(+/-) mice exhibited CIA responses ranging from mild (single digits) to severe swelling of all four paws, while in the few GM-CSF(-/-) mice that developed CIA the disease was confined to single digits. Despite the putative role of GM-CSF in dendritic cell development, GM-CSF-deficient mice exhibited both humoral and cellular (delayed-type hypersensitivity) responses to type II collagen; however, the cellular response was significantly reduced in the GM-CSF-deficient mice compared with the wild-type controls. These findings suggest that GM-CSF is required for CIA development in mice and support the idea that GM-CSF is a key cytokine in inflammatory joint disease.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
161
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
3639-44
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:9759887-Animals,
pubmed-meshheading:9759887-Arthritis, Experimental,
pubmed-meshheading:9759887-Collagen,
pubmed-meshheading:9759887-Female,
pubmed-meshheading:9759887-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:9759887-Hypersensitivity, Delayed,
pubmed-meshheading:9759887-Immunity, Innate,
pubmed-meshheading:9759887-Immunoglobulin G,
pubmed-meshheading:9759887-Male,
pubmed-meshheading:9759887-Mice,
pubmed-meshheading:9759887-Mice, Inbred C57BL,
pubmed-meshheading:9759887-Mice, Knockout
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
Protection from collagen-induced arthritis in granulocyte-macrophage colony-stimulating factor-deficient mice.
|
| pubmed:affiliation |
Department of Medicine, Inflammation Research Center, University of Melbourne, Victoria, Australia. campbell@wehi.edu.au
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|