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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
1998-10-22
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pubmed:abstractText |
IL-4 is a pleiotropic cytokine that is essential for the differentiation of Th2 cells and is critically involved in the pathogenesis of certain infectious and allergic diseases. We have produced and functionally characterized a mutant of murine IL-4 (IL-4.Y119D) as a potential antagonist of IL-4. The analysis of IL-4R binding revealed no differences between wild-type and mutated IL-4. Despite this finding, IL-4.Y119D was unable to induce proliferation of several IL-4-responsive T cell lines mediated via the type I IL-4R (IL-4Ralpha/common gamma chain (gamma c chain)) and specifically inhibited the proliferative effect of wild-type IL-4. In contrast, with IL-4.Y119D we found induction of MHC class II and CD23 molecules on resting splenic B cells as well as proliferation of B9 plasmocytoma cells. In addition, IL-4.Y119D induced mRNA for soluble IL-4R, leading to the release of soluble IL-4R protein by spleen cells. In macrophages, mutated IL-4 in combination with IFN-gamma induced TNF-alpha-dependent killing of Leishmania major parasites such as wild-type IL-4. The agonistic effects of IL-4.Y119D were observed on cells expressing the IL-13R alpha-chain, including an IL-13R alpha-chain transfected T cell line, but were absent in T cells that lack this molecule, indicating that IL-4.Y119D conveys its activity via the type II IL-4R (IL-4Ralpha/IL-13Ralpha). The described IL-4 mutant, therefore, represents a new tool to use in dissecting different IL-4 functions that are mediated by either type I or type II IL-4R complexes.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Aspartic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Histocompatibility Antigens Class II,
http://linkedlifedata.com/resource/pubmed/chemical/Il13ra1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-13,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-13 Receptor alpha1...,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, IgE,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-13,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/Tyrosine
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
161
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3484-92
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9759868-Amino Acid Substitution,
pubmed-meshheading:9759868-Animals,
pubmed-meshheading:9759868-Aspartic Acid,
pubmed-meshheading:9759868-B-Lymphocytes,
pubmed-meshheading:9759868-Cell Division,
pubmed-meshheading:9759868-Cell Line,
pubmed-meshheading:9759868-Cytotoxicity, Immunologic,
pubmed-meshheading:9759868-Female,
pubmed-meshheading:9759868-Growth Substances,
pubmed-meshheading:9759868-Histocompatibility Antigens Class II,
pubmed-meshheading:9759868-Interleukin-13,
pubmed-meshheading:9759868-Interleukin-13 Receptor alpha1 Subunit,
pubmed-meshheading:9759868-Interleukin-4,
pubmed-meshheading:9759868-Interphase,
pubmed-meshheading:9759868-Leishmania major,
pubmed-meshheading:9759868-Lymphocyte Activation,
pubmed-meshheading:9759868-Macrophages,
pubmed-meshheading:9759868-Mice,
pubmed-meshheading:9759868-Mice, Inbred BALB C,
pubmed-meshheading:9759868-Mice, Inbred C57BL,
pubmed-meshheading:9759868-Mice, Knockout,
pubmed-meshheading:9759868-Mutagenesis, Site-Directed,
pubmed-meshheading:9759868-Protein Binding,
pubmed-meshheading:9759868-Receptors, IgE,
pubmed-meshheading:9759868-Receptors, Interleukin,
pubmed-meshheading:9759868-Receptors, Interleukin-13,
pubmed-meshheading:9759868-Receptors, Interleukin-4,
pubmed-meshheading:9759868-Solubility,
pubmed-meshheading:9759868-T-Lymphocytes,
pubmed-meshheading:9759868-Transfection,
pubmed-meshheading:9759868-Tumor Cells, Cultured,
pubmed-meshheading:9759868-Tyrosine
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pubmed:year |
1998
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pubmed:articleTitle |
Specific antagonism of type I IL-4 receptor with a mutated form of murine IL-4.
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pubmed:affiliation |
Institut für Klinische Mikrobiologie, Immunologie, und Hygiene der Universität Erlangen-Nürnberg, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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