Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
8
|
| pubmed:dateCreated |
1998-10-29
|
| pubmed:abstractText |
Recently we found that a single administration of T-2 toxin (T-2), a trichothecene mycotoxin, into mice induced DNA fragmentation, a biochemical hallmark of apoptosis, in the thymus. In this study, we investigated the effective chemical structure(s) of T-2-derived metabolites capable of inducing thymic apoptosis in vivo in mice. Metabolic conversion of T-2 to 3'-hydroxy-T-2 toxin (3'-OH-T-2) did not diminish the apoptosis-inducing activity, since essentially the same level of fragmented DNA was detected in the thymus taken from mice injected with either T-2 or 3'-OH-T-2. In contrast, hydrolysis of T-2 and 3'-OH-T-2 at the carbon-4 (C-4) position to HT-2 toxin (HT-2) and 3'-hydroxy-HT-2 toxin (3'-OH-HT-2), respectively, greatly decreased the level of DNA fragmentation. Similarly, hydrolysis of T-2 at the carbon-8 (C-8) position to neosolaniol strongly diminished its ability to induce DNA fragmentation. T-2 tetraol, having no ester groups, was unable to induce apoptosis. Based on the data presented in this study, we concluded that both the acetyl group at the C-4 position and the isovaleryl or 3'-hydroxyisovaleryl group at the C-8 position of the T-2 molecule are important for inducing cell death through apoptosis in the thymus.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0916-8451
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
62
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1492-7
|
| pubmed:dateRevised |
2003-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:9757554-Animals,
pubmed-meshheading:9757554-Apoptosis,
pubmed-meshheading:9757554-Chromatography, Gas,
pubmed-meshheading:9757554-Colorimetry,
pubmed-meshheading:9757554-DNA Fragmentation,
pubmed-meshheading:9757554-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:9757554-Female,
pubmed-meshheading:9757554-Flow Cytometry,
pubmed-meshheading:9757554-Fusarium,
pubmed-meshheading:9757554-Mice,
pubmed-meshheading:9757554-Mice, Inbred BALB C,
pubmed-meshheading:9757554-Phenotype,
pubmed-meshheading:9757554-Random Allocation,
pubmed-meshheading:9757554-Structure-Activity Relationship,
pubmed-meshheading:9757554-T-2 Toxin,
pubmed-meshheading:9757554-T-Lymphocytes,
pubmed-meshheading:9757554-Thymus Gland,
pubmed-meshheading:9757554-Time Factors
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
Structure-function relationship of T-2 toxin and its metabolites in inducing thymic apoptosis in vivo in mice.
|
| pubmed:affiliation |
Faculty of Agriculture, Kagawa University, Japan.
|
| pubmed:publicationType |
Journal Article
|