Linked Life Data

9757093

Source:http://linkedlifedata.com/resource/pubmed/id/9757093

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 5
pubmed:dateCreated
1998-12-14
pubmed:abstractText
The positions of eight Se atoms in a selenomethionyl 35 kDa protein were determined at 2.0 and 2.5 A resolution using the direct-methods program SnB. Data at the selenium peak, edge and remote wavelengths were measured and processed independently. Anomalous difference E magnitudes at each wavelength were derived by two different procedures: renormalized diffE values were calculated according to the equation diffE = ¿sigma[(f + f')2 + f"2]||E+|-|E-||¿1/2/2q(sigma f"2)1/2, where q is a least-squares fitted renormalization function of sinstraight theta/lambda such that <diffE2> = 1.0; and difference E magnitudes were calculated from DeltaF2. Locally normalized E magnitudes corresponding to |FA| were also derived from the combination of the data at all three wavelengths through the use of the MADSYS program suite. Each of the independent sets of anomalous difference E magnitudes was capable of producing the correct solution, as did the E data obtained from the FA data. Higher success rates with SnB were observed for the 2.0 A peak and edge diffE data.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0907-4449
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
54
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
799-804
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
The use of SnB to determine an anomalous scattering substructure.
pubmed:affiliation
Hauptman-Woodward Medical Research Institute, 73 High Street, Buffalo, NY 14203, USA, and Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, NY 14263, USA. smith@hwi.buffalo.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.