Linked Life Data

975541

Source:http://linkedlifedata.com/resource/pubmed/id/975541

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1976-12-30
pubmed:abstractText
Lactate can be determined rapidly in blood by spectrophotometric and amperometric (enzyme electrode) procedures based on its oxidation by ferricyanide, the reaction being catalyzed with yeast L(+)-lactate dehydrogenase (cytochrome b2) (EC 1.1.2.3). In the photometric method lactate can be measured in a few minutes, but blood samples must first be deproteinized. In the amperometric procedure no treatment of blood is needed except ferricyanide addition. The enzyme electrode we used has a response time shorter than 1 min when its critical variables are optimized. Preliminary standardization is reduced to minimum operation, because electrode response is proportional to lactate concentration over a wide range (0.1 to 8.0 mol/liter) and many determinations can be done with little cost in enzyme. A simple electrical device ("two-electrode device") is described that is well suited for furture micro-cell construction. Lactate determinations on a series of normal blood samples show no deviation between results by these new methods and the usual ultraviolet spectrophotometric lactate tests.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0009-9147
pubmed:author
pubmed:issnType
Print
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1802-5
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1976
pubmed:articleTitle
Spectrophotometric and electrochemical determinations of L(+)-lactate in blood by use of lactate dehydrogenase from yeast.
pubmed:publicationType
Journal Article, Comparative Study