Linked Life Data

9755235

Source:http://linkedlifedata.com/resource/pubmed/id/9755235

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1998-11-5
pubmed:abstractText
Macrophage metalloelastase, a member of the human matrix metalloproteinase family, is believed to play an important role in angiostatin generation, which, in experimental studies, has an antiangiogenic function and is a key molecule in tumor dormancy. However, no clinical studies have been reported regarding the correlation between human macrophage metalloelastase (HME) gene expression and angiostatin production. Therefore, the present study was designed to evaluate the HME messenger RNA (mRNA) expression and angiostatin generation in hepatocellular carcinoma (HCC). Tumorous and contiguous nontumorous tissues were obtained from 40 HCC patients who underwent curative partial hepatectomy. By using Northern blot hybridization, HME mRNA was detected in 25 of the 40 HCC samples and, in all of these cases, the expression in tumorous tissues was stronger than in the nontumorous tissues. In situ hybridization identified the HCC cells as mainly responsible for the signals shown in Northern blot. Angiostatin was detected by Western blot mainly in tumors and showed significant association with HME mRNA expression in tumorous tissues (P = .0008). The patients whose tumors did not express HME mRNA and, thus, did not produce angiostatin, demonstrated poorer survival than those whose tumors showed high expression of HME mRNA and angiostatin generation (P = . 002). The univariate and multivariate analyses revealed that HME mRNA expression is a new and independent variable affecting overall survival (P = .001 and P = .03, respectively). These findings show that the HME gene is expressed in HCC being significantly associated with angiostatin generation by such tumors and that HME mRNA expression may serve as a new molecular prognostic marker in HCC patients after partial hepatectomy.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0270-9139
pubmed:author
pubmed:issnType
Print
pubmed:volume
28
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
986-93
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:9755235-Adult, pubmed-meshheading:9755235-Aged, pubmed-meshheading:9755235-Angiostatins, pubmed-meshheading:9755235-Carcinoma, Hepatocellular, pubmed-meshheading:9755235-DNA Primers, pubmed-meshheading:9755235-DNA Probes, pubmed-meshheading:9755235-Female, pubmed-meshheading:9755235-Gene Expression Regulation, Enzymologic, pubmed-meshheading:9755235-Gene Expression Regulation, Neoplastic, pubmed-meshheading:9755235-Hepatectomy, pubmed-meshheading:9755235-Hepatitis B Surface Antigens, pubmed-meshheading:9755235-Hepatitis C Antibodies, pubmed-meshheading:9755235-Humans, pubmed-meshheading:9755235-Liver, pubmed-meshheading:9755235-Liver Neoplasms, pubmed-meshheading:9755235-Male, pubmed-meshheading:9755235-Matrix Metalloproteinase 12, pubmed-meshheading:9755235-Metalloendopeptidases, pubmed-meshheading:9755235-Middle Aged, pubmed-meshheading:9755235-Peptide Fragments, pubmed-meshheading:9755235-Plasminogen, pubmed-meshheading:9755235-RNA, Messenger, pubmed-meshheading:9755235-Retrospective Studies, pubmed-meshheading:9755235-Survival Analysis, pubmed-meshheading:9755235-Time Factors, pubmed-meshheading:9755235-Transcription, Genetic
pubmed:year
1998
pubmed:articleTitle
Expression of human macrophage metalloelastase gene in hepatocellular carcinoma: correlation with angiostatin generation and its clinical significance.
pubmed:affiliation
First Department of Surgery, Faculty of Medicine, Kyoto University, Kyoto, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't