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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1998-12-21
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pubmed:abstractText |
Synthetic DNA has been used to introduce variability into protein-coding regions. In protocols that produce a few mutations per gene, the sampling of amino-acid sequence space is limited by the bias imposed by the genetic code. It has long been apparent that the incorporation of trinucleotides in the synthetic regime would circumvent this problem and significantly enhance the usefulness of the technique.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
|
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1074-5521
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:volume |
5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
519-27
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pubmed:dateRevised |
2008-8-29
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pubmed:meshHeading |
pubmed-meshheading:9751646-Cloning, Molecular,
pubmed-meshheading:9751646-Codon,
pubmed-meshheading:9751646-DNA,
pubmed-meshheading:9751646-Deoxyribonucleases, Type II Site-Specific,
pubmed-meshheading:9751646-Magnetic Resonance Spectroscopy,
pubmed-meshheading:9751646-Models, Chemical,
pubmed-meshheading:9751646-Mutagenesis, Site-Directed,
pubmed-meshheading:9751646-Oligonucleotides
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pubmed:year |
1998
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pubmed:articleTitle |
Combination of DMT-mononucleotide and Fmoc-trinucleotide phosphoramidites in oligonucleotide synthesis affords an automatable codon-level mutagenesis method.
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pubmed:affiliation |
Department of Molecular Recognition and Biostructure, Instituto de Biotecnología/UNAM, Cuernavaca, Mor. México.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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