Linked Life Data

9751212

Source:http://linkedlifedata.com/resource/pubmed/id/9751212

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1998-10-14
pubmed:abstractText
Leptin is a 16-kDa protein synthesized in adipose tissue that produces a satiety effect in the CNS. Leptin may gain access to the brain via receptor-mediated transport through the blood-brain barrier (BBB), and the BBB leptin receptor (OBR) may regulate the availability of circulating leptin to brain cells. The aim of the present study was twofold: first, to identify the OBR isoform expressed at the BBB, i.e., short, or "a," and long, or "b," form; and second, to compare the abundance of the BBB OBR mRNA and protein between control and high fat-fed rats. RT-PCR with isoform-specific primers showed that OBRa is the most abundant isoform at the BBB. BBB OBRa transcript content was markedly increased in high fat-fed rats compared with controls (11-fold), and no changes were observed in the expression of the internal standard control actin. The high fat feeding induction of OBR mRNA was correlated with an increase in the immunoreactive BBB OBR determined by immunocytochemistry using an all-isoform reactive antibody in high fat-fed obese rats. This investigation demonstrates (a) the OBRa is the principal leptin receptor expressed at the BBB and (b) this BBB OBR isoform is up-regulated by a high fat diet.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0022-3042
pubmed:author
pubmed:issnType
Print
pubmed:volume
71
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1761-4
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Up-regulation of blood-brain barrier short-form leptin receptor gene products in rats fed a high fat diet.
pubmed:affiliation
Department of Medicine, UCLA School of Medicine, Los Angeles, California 90095, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't