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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1998-10-23
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pubmed:databankReference |
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pubmed:abstractText |
The molecular dissection of desiccation tolerance in the resurrection plant Craterostigma plantagineum led to the isolation of two dehydration-stress inducible homeo-domain-leucine zipper genes (CPHB-1 and -2). When the coding region of CPHB-1 was used as bait in the yeast two-hybrid system, the ability of CPHB-1 to form homodimers was demonstrated. The two-hybrid system was also used to isolate CPHB-2, which heterodimerises with CPHB-1. Both transcripts are inducible by dehydration in leaves and roots, but steady state levels vary in response to exogenously applied ABA. Although expression of CPHB-1 is not inducible by ABA, the transcript level of CPHB-2 increases during ABA-treatment. Both genes are expressed at very early stages of dehydration and thus may be involved in the regulation of gene expression during dehydration. CPHB-1 and -2 differential expression in response to ABA suggests that they act in different branches of the dehydration-induced signalling network. In vitro binding studies revealed that CPHB-1 specifically binds to the pseudopalindromic sequence CAAT(C/G)ATTG. Using this element for in vitro binding studies with nuclear proteins from dehydrated leaves, an inducible DNA-protein complex was identified.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Abscisic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Plant,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Homeodomain Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Plant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Plant
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0960-7412
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
15
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
413-21
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9750352-Abscisic Acid,
pubmed-meshheading:9750352-Amino Acid Sequence,
pubmed-meshheading:9750352-Angiosperms,
pubmed-meshheading:9750352-Base Sequence,
pubmed-meshheading:9750352-Binding Sites,
pubmed-meshheading:9750352-Cloning, Molecular,
pubmed-meshheading:9750352-DNA, Complementary,
pubmed-meshheading:9750352-DNA, Plant,
pubmed-meshheading:9750352-DNA Primers,
pubmed-meshheading:9750352-Dimerization,
pubmed-meshheading:9750352-Gene Expression Regulation, Plant,
pubmed-meshheading:9750352-Genes, Homeobox,
pubmed-meshheading:9750352-Genes, Plant,
pubmed-meshheading:9750352-Homeodomain Proteins,
pubmed-meshheading:9750352-Leucine Zippers,
pubmed-meshheading:9750352-Molecular Sequence Data,
pubmed-meshheading:9750352-Multigene Family,
pubmed-meshheading:9750352-Plant Proteins,
pubmed-meshheading:9750352-Polymerase Chain Reaction,
pubmed-meshheading:9750352-RNA, Messenger,
pubmed-meshheading:9750352-RNA, Plant
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pubmed:year |
1998
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pubmed:articleTitle |
Two dehydration-inducible transcripts from the resurrection plant Craterostigma plantagineum encode interacting homeodomain-leucine zipper proteins.
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pubmed:affiliation |
Max-Planck-Institut für Züchtungsforschung, Köln, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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