Linked Life Data

9747802

Source:http://linkedlifedata.com/resource/pubmed/id/9747802

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1998-10-9
pubmed:databankReference
pubmed:abstractText
We have previously identified two cDNAs encoding vegetative storage proteins (VSPs) in Arabidopsis thaliana. Unlike soybean in which VSPs accumulate at high levels in leaves, A. thaliana VSP mRNAs are abundant in flowers. To understand tissue-specific expression and possible roles of VSPs on reproductive organ development, genes corresponding to VSPs (Vsp1 and Vsp2) and their putative promoters were characterized in this study. Genomic sequence analysis revealed that Vsp1 and Vsp2 resemble each other except in their introns, and that these two genes were organized in a tandem array with an interval of 6 kb in a region. The expression patterns of Vsp1 and Vsp2 were examined using transgenic A. thaliana plants carrying a promoter from Vsp1 or Vsp2 fused to a bacterial beta-glucuronidase (GUS) reporter gene. The promoter from Vsp1 expressed its effect in gynoecia, especially in styles, the basal and distal ends of ovaries and in siliques, whereas the promoter from Vsp2 showed its activity in vegetative shoots, petioles, peduncles and receptacles of floral organs. These results suggest that expression of Vsp1 and Vsp2 may be developmentally regulated in A. thaliana. In the transgenic plants, the GUS activity was induced by wounding in an area around the mid-rib of leaves. Therefore, Vsp1 and Vsp2 promoters appear to have elements required for both tissue specificity and wounding.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0167-4412
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
38
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
565-76
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:9747802-Arabidopsis, pubmed-meshheading:9747802-Base Sequence, pubmed-meshheading:9747802-Chromosome Mapping, pubmed-meshheading:9747802-DNA Primers, pubmed-meshheading:9747802-Gene Expression Regulation, Developmental, pubmed-meshheading:9747802-Gene Expression Regulation, Plant, pubmed-meshheading:9747802-Genes, Plant, pubmed-meshheading:9747802-Genes, Reporter, pubmed-meshheading:9747802-Glucuronidase, pubmed-meshheading:9747802-Molecular Sequence Data, pubmed-meshheading:9747802-Plant Proteins, pubmed-meshheading:9747802-Plants, Genetically Modified, pubmed-meshheading:9747802-Promoter Regions, Genetic, pubmed-meshheading:9747802-RNA, Messenger, pubmed-meshheading:9747802-RNA, Plant, pubmed-meshheading:9747802-Sequence Homology, Nucleic Acid, pubmed-meshheading:9747802-Tissue Distribution
pubmed:year
1998
pubmed:articleTitle
Arabidopsis thaliana vegetative storage protein (VSP) genes: gene organization and tissue-specific expression.
pubmed:affiliation
Research Institute for Bioresources, Okayama University, Kurashiki, Japan.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't