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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1998-12-7
|
| pubmed:abstractText |
The changes in glutathione-independent prostaglandin D2 synthetase (PGD-S) during maturation in the rat were determined in selected organs by an RIA using PGD-S purified from rat cerebrospinal fluid and a monospecific anti-rat PGD-S polyclonal antibody. In a survey of its tissue distribution in various organ extracts and biological fluids, it was found that the concentration of PGD-S was highest in the epididymis-about 6- and 80-fold greater than that in the brain and testis, respectively. During maturation, PGD-S concentration increased steadily in the testis and epididymis; this is in contrast to the pattern of changes in the brain and liver, which showed a general trend of decline. Reverse transcription-polymerase chain reaction and Southern blotting were used to demonstrate the presence of PGD-S mRNA transcript in the testis and in Sertoli and germ cells. In the epididymis, the steady-state PGD-S mRNA level was highest in the caput, followed by the cauda and corpus. Orchiectomy induced a drastic reduction of PGD-S concentration in all three epididymal compartments. Administration of dihydrotestosterone (DHT) failed to restore the reduced epididymal PGD-S level except in the caput epididymis, where 4 days after DHT treatment the level of PGD-S was restored to about 50% of the pre-orchiectomized level; this suggests that the epididymal PGD-S level is not entirely regulated by androgen and that another yet to be identified testicular factor(s) is likely to be involved in its regulation. Germ cell-conditioned medium was also shown to stimulate PGD-S expression in the Sertoli cell. These results illustrate that PGD-S is an important molecule in testicular and epididymal function and that it is likely involved in spermatogenesis and sperm maturation.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Conditioned,
http://linkedlifedata.com/resource/pubmed/chemical/Indicators and Reagents,
http://linkedlifedata.com/resource/pubmed/chemical/Intramolecular Oxidoreductases,
http://linkedlifedata.com/resource/pubmed/chemical/Lipocalins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/prostaglandin R2 D-isomerase
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0006-3363
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
59
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
843-53
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9746734-Animals,
pubmed-meshheading:9746734-Blotting, Northern,
pubmed-meshheading:9746734-Blotting, Southern,
pubmed-meshheading:9746734-Culture Media, Conditioned,
pubmed-meshheading:9746734-Epididymis,
pubmed-meshheading:9746734-Germ Cells,
pubmed-meshheading:9746734-Indicators and Reagents,
pubmed-meshheading:9746734-Intramolecular Oxidoreductases,
pubmed-meshheading:9746734-Lipocalins,
pubmed-meshheading:9746734-Male,
pubmed-meshheading:9746734-Orchiectomy,
pubmed-meshheading:9746734-RNA, Messenger,
pubmed-meshheading:9746734-Radioimmunoassay,
pubmed-meshheading:9746734-Rats,
pubmed-meshheading:9746734-Rats, Sprague-Dawley,
pubmed-meshheading:9746734-Recombinant Proteins,
pubmed-meshheading:9746734-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:9746734-Seminiferous Epithelium,
pubmed-meshheading:9746734-Sertoli Cells,
pubmed-meshheading:9746734-Testis,
pubmed-meshheading:9746734-Tissue Distribution
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
Rat prostaglandin D2 synthetase: its tissue distribution, changes during maturation, and regulation in the testis and epididymis.
|
| pubmed:affiliation |
The Population Council, New York, New York 10021, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|