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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1998-11-16
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pubmed:abstractText |
The function and molecular expression of ATP-sensitive potassium (KATP) channels in murine colonic smooth muscle was investigated by intracellular electrical recording from intact muscles, patch-clamp techniques on isolated smooth muscle myocytes, and reverse transcription polymerase chain reaction (RT-PCR) on isolated cells. Lemakalim (1 microM) caused hyperpolarization of intact muscles (17. 2 +/- 3 mV). The hyperpolarization was blocked by glibenclamide (1-10 microM). Addition of glibenclamide (10 microM) alone resulted in membrane depolarization (9.3 +/- 1.7 mV). Lemakalim induced an outward current of 15 +/- 3 pA in isolated myocytes bathed in 5 mM external K+ solution. Application of lemakalim to cells in symmetrical K+ solutions (140/140 mM) resulted in a 97 +/- 5 pA inward current. Both currents were blocked by glibenclamide (1 microM). Pinacidil (1 microM) also activated an inwardly rectifying current that was insensitive to 4-aminopyridine and barium. In single-channel studies, lemakalim (1 microM) and diazoxide (300 microM) increased the open probability of a 27-pS K+ channel. Openings of these channels decreased with time after patch excision. Application of ADP (1 mM) or ATP (0.1 mM) to the inner surface of the patches reactivated channel openings. The conductance and characteristics of the channels activated by lemakalim were consistent with the properties of KATP. RT-PCR demonstrated the presence of Kir 6.2 and SUR2B transcripts in colonic smooth muscle cells; transcripts for Kir 6.1, SUR1, and SUR2A were not detected. These molecular studies are the first to identify the molecular components of KATP in colonic smooth muscle cells. Together with the electrophysiological experiments, we conclude that KATP channels are expressed in murine colonic smooth muscle cells and suggest that these channels may be involved in dual regulation of resting membrane potential, excitability, and contractility.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9746521-1328618,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9746521-1537097,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9746521-1570027,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9746521-1715132,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9746521-1755851,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9746521-1822556,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9746521-2109832,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9746521-2119205,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9746521-2501478,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9746521-2501869,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9746521-2511995,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9746521-2517114,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9746521-2691645,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9746521-3267155,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9746521-3826336,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9746521-3965340,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9746521-6310409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9746521-7491898,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9746521-7502040,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/9746521-7858882,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9746521-7890693,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/9746521-8733582,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9746521-8762088,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/9746521-8865068,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9746521-8996232,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9746521-9379406,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/9746521-9458709
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0006-3495
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
75
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1793-800
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9746521-Adenosine Diphosphate,
pubmed-meshheading:9746521-Adenosine Triphosphate,
pubmed-meshheading:9746521-Animals,
pubmed-meshheading:9746521-Colon,
pubmed-meshheading:9746521-Cromakalim,
pubmed-meshheading:9746521-Female,
pubmed-meshheading:9746521-Glyburide,
pubmed-meshheading:9746521-Ion Channel Gating,
pubmed-meshheading:9746521-Male,
pubmed-meshheading:9746521-Membrane Potentials,
pubmed-meshheading:9746521-Mice,
pubmed-meshheading:9746521-Mice, Inbred BALB C,
pubmed-meshheading:9746521-Muscle, Smooth,
pubmed-meshheading:9746521-Patch-Clamp Techniques,
pubmed-meshheading:9746521-Potassium Channels,
pubmed-meshheading:9746521-RNA, Messenger,
pubmed-meshheading:9746521-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:9746521-Transcription, Genetic
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pubmed:year |
1998
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pubmed:articleTitle |
Basal activation of ATP-sensitive potassium channels in murine colonic smooth muscle cell.
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pubmed:affiliation |
Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, Nevada 89557 USA.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.
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