Linked Life Data

9745892

Source:http://linkedlifedata.com/resource/pubmed/id/9745892

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1999-1-12
pubmed:abstractText
In our previous paper (Belikov et al., (1993), Nucl. Acids Res., 21, 4796-4802) we had studied DNA-protein architecture of so-called Alu-repeats in D. melanogaster ribosomal nontranscribed spacer using DNase I genomic footprinting and UV-induced DNA-protein crosslinking. Our data indicated precise positioning of two non-histone proteins (rABP50 and rABP 70), histone octamer, and histone H1 within the sequences of Alu-repeats. The data on the histone H1 binding sites within Alu-repeat region was presented, but not discussed as the authors could not reach a consensus in its evaluation. Here, the authors use these data to present a model of placement of the linker histone(s) within nucleosome. Our model places one contact of the globular domain of linker histone in the major groove on the inside of DNA superhelix just within the end of the core particle (site +6.5) and the second, close to the dyad (site -1). C-terminal tail binds to the internucleosomal spacer and N-terminal tail interacts simultaneously with the adjacent gyres thus stabilizing DNA superhelix.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0739-1102
pubmed:author
pubmed:issnType
Print
pubmed:volume
16
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
35-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Localization of histone H1 binding sites within the nucleosome by UV-induced H1-DNA crosslinking in vivo.
pubmed:affiliation
W.A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Laboratory of Structure and Function of Chromatin, Moscow.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't