|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
6
|
|
pubmed:dateCreated |
1998-10-6
|
|
pubmed:abstractText |
We have shown that an estradiol-dependent activation of human T cell leukemia virus-I Tax leads to the inhibition of cell proliferation and to the induction of apoptosis. The present study demonstrates that a hormone-dependent activation of Tax promotes an enhanced prooxidant state in stably transfected Jurkat cells as measured by changes in the intracellular levels of glutathione and H2O2; these changes are followed by apoptotic cell death. Additional stimulation of the CD3/TCR pathway enhances the oxidative and apoptotic effects. Both Tax-mediated apoptosis and oxidative stress can be potently suppressed by antioxidants, as is seen with the administration of recombinant thioredoxin (adult T cell leukemia-derived factor) or pyrrolidine dithiocarbamate. Hormone-induced Tax activation induces a long-lasting activation of NF-kappaB, which is a major target of reactive oxygen intermediates. The long-term exposure of Jurkat cells to hormone eventually results in a selection of cell clones that have lost Tax activity. A subsequent transfection of these apparently "nonresponsive" clones allows the recovery of Tax responses in these cells. Our observations indicate that changes in the intracellular redox status may be a determining factor in Tax-mediated DNA damage, apoptosis, and selection against the long-term expression of Tax function.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
AIM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD3,
http://linkedlifedata.com/resource/pubmed/chemical/Antioxidants,
http://linkedlifedata.com/resource/pubmed/chemical/Estradiol,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, tax,
http://linkedlifedata.com/resource/pubmed/chemical/Oxidants,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor-CD3 Complex, Antigen...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Estrogen,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Sep
|
|
pubmed:issn |
0022-1767
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
15
|
|
pubmed:volume |
161
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
3050-5
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:9743370-Antibodies, Monoclonal,
pubmed-meshheading:9743370-Antigens, CD3,
pubmed-meshheading:9743370-Antioxidants,
pubmed-meshheading:9743370-Apoptosis,
pubmed-meshheading:9743370-Drug Synergism,
pubmed-meshheading:9743370-Estradiol,
pubmed-meshheading:9743370-Gene Products, tax,
pubmed-meshheading:9743370-Human T-lymphotropic virus 1,
pubmed-meshheading:9743370-Humans,
pubmed-meshheading:9743370-Intracellular Fluid,
pubmed-meshheading:9743370-Jurkat Cells,
pubmed-meshheading:9743370-Lymphocyte Activation,
pubmed-meshheading:9743370-Oxidants,
pubmed-meshheading:9743370-Oxidation-Reduction,
pubmed-meshheading:9743370-Receptor-CD3 Complex, Antigen, T-Cell,
pubmed-meshheading:9743370-Receptors, Estrogen,
pubmed-meshheading:9743370-Recombinant Fusion Proteins,
pubmed-meshheading:9743370-T-Lymphocytes,
pubmed-meshheading:9743370-Time Factors,
pubmed-meshheading:9743370-Transfection
|
|
pubmed:year |
1998
|
|
pubmed:articleTitle |
Human T cell leukemia virus-I (HTLV-I) Tax-mediated apoptosis in activated T cells requires an enhanced intracellular prooxidant state.
|
|
pubmed:affiliation |
Department of Internal Medicine I, Eberhard-Karls University, Tübingen, Germany.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
