Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions |
umls-concept:C0035647,
umls-concept:C0039194,
umls-concept:C0079189,
umls-concept:C0302350,
umls-concept:C0332307,
umls-concept:C0333051,
umls-concept:C0376659,
umls-concept:C0871261,
umls-concept:C1517476,
umls-concept:C1704632,
umls-concept:C1706817,
umls-concept:C1979963,
umls-concept:C2003903,
umls-concept:C2911692
|
pubmed:issue |
6
|
pubmed:dateCreated |
1998-10-6
|
pubmed:abstractText |
Vaccination with a poorly immunogenic/nonimmunogenic tumor fails to protect the host from a subsequent challenge with the same tumor. The mechanisms underlying the failure of these tumors to sensitize therapeutic T cells are not clearly understood, but the inability of host T cells to recognize tumor has been implicated. In this study, vaccination with the poorly immunogenic B16BL6-D5 (D5 H-2b) tumor fails to generate therapeutic T cells from the tumor vaccine-draining lymph nodes (TVDLN) in our adoptive immunotherapy model. However, if vaccination is performed with an allogeneic MHC class I gene (H-2 Kd)-modified tumor, the T cells obtained from the TVDLN are therapeutic after activation with anti-CD3 and IL-2. Lymph nodes (LN) draining both D5 and D5-Kd tumor vaccines contained increased numbers of cells with reduced expression of L-selectin (L-selectin(low/-)) compared with naive LN. This implies that vaccination led to sensitization of T cells even in LN draining the unmodified D5 tumor. L-selectin(low/-) cells from D5-Kd, but not D5, TVDLN were therapeutic in our animal model. No antitumor activity was seen in the high level L-selectin T cells. L-selectin(low/-) T cells exhibited tumor-specific cytokine release that was type 2 (IL-4, IL-10) following vaccination with native D5 and type 1 (IFN-gamma) following vaccination with gene-modified D5-Kd. Our data suggest that the failure of unmodified D5 to generate therapeutic T cells is not due to an inability to recognize tumor Ags, but, rather, to the induction of an immune response that is ineffective in mediating tumor regression, i.e., immune deviation.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
AIM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD3,
http://linkedlifedata.com/resource/pubmed/chemical/Cancer Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/L-Selectin,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, DNA
|
pubmed:status |
MEDLINE
|
pubmed:month |
Sep
|
pubmed:issn |
0022-1767
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
15
|
pubmed:volume |
161
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
3033-41
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:9743368-Animals,
pubmed-meshheading:9743368-Antibodies, Monoclonal,
pubmed-meshheading:9743368-Antigens, CD3,
pubmed-meshheading:9743368-Cancer Vaccines,
pubmed-meshheading:9743368-Cell Separation,
pubmed-meshheading:9743368-Cytokines,
pubmed-meshheading:9743368-Cytomegalovirus,
pubmed-meshheading:9743368-Female,
pubmed-meshheading:9743368-Genes, MHC Class I,
pubmed-meshheading:9743368-Immunotherapy, Adoptive,
pubmed-meshheading:9743368-Interleukin-2,
pubmed-meshheading:9743368-L-Selectin,
pubmed-meshheading:9743368-Lymph Nodes,
pubmed-meshheading:9743368-Melanoma,
pubmed-meshheading:9743368-Mice,
pubmed-meshheading:9743368-Mice, Inbred C57BL,
pubmed-meshheading:9743368-Th1 Cells,
pubmed-meshheading:9743368-Th2 Cells,
pubmed-meshheading:9743368-Tumor Cells, Cultured,
pubmed-meshheading:9743368-Vaccines, DNA
|
pubmed:year |
1998
|
pubmed:articleTitle |
Gene-modified tumor vaccine with therapeutic potential shifts tumor-specific T cell response from a type 2 to a type 1 cytokine profile.
|
pubmed:affiliation |
The Robert W. Franz Cancer Research Center, The Earle A. Chiles Research Institute, Portland, OR 97213, USA.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|