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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1998-10-6
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pubmed:abstractText |
Posttranslational processing of chemokines increases (IL-8) or decreases (monocyte chemotactic protein-1) their chemotactic potency. Macrophage-derived chemokine (MDC) attracts monocytes, dendritic cells, activated lymphocytes, and NK cells and has reportedly anti-HIV-1 activity. Here we report that truncation of MDC by deletion of two NH2-terminal residues resulted in impaired binding to CC chemokine receptor (CCR)4, the only identified MDC receptor so far. Truncated MDC(3-69) failed to desensitize calcium mobilization by MDC(1-69) or thymus- and activation-regulated chemokine (TARC), another CCR4 ligand. MDC(3-69) lacked HUT-78 T cell chemotactic activity but retained its capacity to attract monocytes and to desensitize chemotaxis. Compared with MDC(1-69), MDC(3-69) had weak but enhanced antiviral activity against M- and T-tropic HIV-1 strains. Furthermore, both MDC forms failed to signal through the orphan receptors Bonzo/STRL33 and BOB/GPR15 and to desensitize RANTES and stromal cell-derived factor (SDF)-1 responses in CCR5-transfected and CXC chemokine receptor (CXCR)4-transfected cells, respectively. These findings suggest that MDC recognizes another, yet unidentified, receptor. We conclude that minimal NH2-terminal truncation of MDC differentially affects its various immunologic functions.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adjuvants, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Anti-HIV Agents,
http://linkedlifedata.com/resource/pubmed/chemical/CCL22 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/CCR4 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Ccl22 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Ccr4 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CCL22,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokines, CC,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, CCR4,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Chemokine,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
161
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2672-5
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:9743322-Adjuvants, Immunologic,
pubmed-meshheading:9743322-Animals,
pubmed-meshheading:9743322-Anti-HIV Agents,
pubmed-meshheading:9743322-Chemokine CCL22,
pubmed-meshheading:9743322-Chemokines, CC,
pubmed-meshheading:9743322-Chemotaxis, Leukocyte,
pubmed-meshheading:9743322-HIV-1,
pubmed-meshheading:9743322-Humans,
pubmed-meshheading:9743322-Lymphocytes,
pubmed-meshheading:9743322-Mice,
pubmed-meshheading:9743322-Monocytes,
pubmed-meshheading:9743322-Peptide Fragments,
pubmed-meshheading:9743322-Receptors, CCR4,
pubmed-meshheading:9743322-Receptors, Chemokine,
pubmed-meshheading:9743322-Recombinant Proteins,
pubmed-meshheading:9743322-Signal Transduction,
pubmed-meshheading:9743322-Tumor Cells, Cultured
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pubmed:year |
1998
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pubmed:articleTitle |
Enhanced anti-HIV-1 activity and altered chemotactic potency of NH2-terminally processed macrophage-derived chemokine (MDC) imply an additional MDC receptor.
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pubmed:affiliation |
Laboratory of Molecular Immunology, Rega Institute for Medical Research, University of Leuven, Belgium.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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