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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
11
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pubmed:dateCreated |
1977-1-3
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pubmed:abstractText |
We have developed an effective experimental system for the characterization of molecular and structural mobility. It incorporates a modified fluorescence microscope geometry and a variety of analytical techniques to measure effective diffusion coefficients ranging over almost six orders of magnitude, from less than 10(-11) cm2/s to greater than 10(-6) cm2/s. Two principal techniques, fluorescence correlation spectroscopy (FCS) and fluorescence photobleaching recovery (FPR), are employed. In the FPR technique, translational transport rates are measured by monitoring the evolution of a spatial inhomogeneity of fluorescence that is produced photochemically in a microscopic volume by a short burst of intense laser radiation. In contrast, FCS uses laser-induced fluorescence to probe the spontaneous concentration fluctuations in microscopic sample volumes. The kinetics are analyzed by computing time-correlation functions of the stochastic fluctuations of the measured fluorescence intensity. The optical system and digital photocount correlator designed around a dedicated minicomputer are described and discussed. The general power of these techniques is demonstrated with examples from studies conducted on bulk solutions, lipid bilayer membranes, and mammalian cell plasma membranes.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/974223-1065895,
http://linkedlifedata.com/resource/pubmed/commentcorrection/974223-1098556,
http://linkedlifedata.com/resource/pubmed/commentcorrection/974223-1246629,
http://linkedlifedata.com/resource/pubmed/commentcorrection/974223-177080,
http://linkedlifedata.com/resource/pubmed/commentcorrection/974223-4429678,
http://linkedlifedata.com/resource/pubmed/commentcorrection/974223-4603215,
http://linkedlifedata.com/resource/pubmed/commentcorrection/974223-4818131,
http://linkedlifedata.com/resource/pubmed/commentcorrection/974223-786399,
http://linkedlifedata.com/resource/pubmed/commentcorrection/974223-822421
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0006-3495
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
16
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1315-29
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:974223-Biological Transport,
pubmed-meshheading:974223-Cell Membrane,
pubmed-meshheading:974223-Diffusion,
pubmed-meshheading:974223-Lasers,
pubmed-meshheading:974223-Membrane Lipids,
pubmed-meshheading:974223-Membrane Proteins,
pubmed-meshheading:974223-Membranes, Artificial,
pubmed-meshheading:974223-Spectrometry, Fluorescence
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pubmed:year |
1976
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pubmed:articleTitle |
Dynamics of fluorescence marker concentration as a probe of mobility.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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