Linked Life Data

9742159

Source:http://linkedlifedata.com/resource/pubmed/id/9742159

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
19
pubmed:dateCreated
1998-10-9
pubmed:abstractText
Axon regeneration requires that injured neurons reinitiate long-distance growth and upregulate specific genes. To address the question of whether inhibitory environmental cues along the axon could exert a negative, tonic downregulation of growth-associated genes, we have examined adult rat Purkinje cells, which are endowed with poor regenerative capabilities. First we have compared their response to axotomy with that of neurons of the inferior olive, lateral reticular nucleus, and deep cerebellar nuclei, all of which vigorously regenerate into growth-permissive transplants. These injured neurons upregulate the transcription factors c-Jun and JunD, GAP-43, and NADPH diaphorase. In contrast, most axotomized Purkinje cells fail to express any of these markers, showing that the strength of this response parallels the regenerative potential of the examined neuron populations. However, strong upregulation of the same genes can be induced in Purkinje cells after colchicine injection into the uninjured adult cerebellum, indicating that their expression could be controlled by retrograde signals. To assess whether myelin-associated neurite growth inhibitory proteins contribute to this regulation, we applied the neutralizing antibodies IN-1 against one of the main inhibitory components of central myelin (NI-250) either in vivo or in vitro to organotypic cerebellar cultures. Application of IN-1 antibodies induces the upregulation of c-Jun, JunD, and NADPH diaphorase in Purkinje cells, showing that their expression is suppressed constitutively by myelin-associated neurite growth inhibitors. Thus, the inhibitory activity of the IN-1 antigen on axon growth is not restricted to the control of growth cone motility but also involves a retrograde regulation of gene expression in adult central neurons.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0270-6474
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7912-29
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:9742159-Age Factors, pubmed-meshheading:9742159-Animals, pubmed-meshheading:9742159-Antibodies, pubmed-meshheading:9742159-Antibodies, Monoclonal, pubmed-meshheading:9742159-Axonal Transport, pubmed-meshheading:9742159-Axotomy, pubmed-meshheading:9742159-Colchicine, pubmed-meshheading:9742159-Female, pubmed-meshheading:9742159-GAP-43 Protein, pubmed-meshheading:9742159-Gene Expression Regulation, Developmental, pubmed-meshheading:9742159-Genes, Immediate-Early, pubmed-meshheading:9742159-Immunoglobulin Fab Fragments, pubmed-meshheading:9742159-Myelin Sheath, pubmed-meshheading:9742159-NADPH Dehydrogenase, pubmed-meshheading:9742159-Nerve Regeneration, pubmed-meshheading:9742159-Neural Inhibition, pubmed-meshheading:9742159-Neurites, pubmed-meshheading:9742159-Neutralization Tests, pubmed-meshheading:9742159-Pregnancy, pubmed-meshheading:9742159-Proto-Oncogene Proteins c-jun, pubmed-meshheading:9742159-Purkinje Cells, pubmed-meshheading:9742159-Rats, pubmed-meshheading:9742159-Rats, Wistar
pubmed:year
1998
pubmed:articleTitle
Retrograde regulation of growth-associated gene expression in adult rat Purkinje cells by myelin-associated neurite growth inhibitory proteins.
pubmed:affiliation
Department of Neuroscience, University of Turin, I-10125 Turin, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't