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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1998-9-24
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pubmed:abstractText |
Autoantibodies to a 64-kD protein and a 40-kD tryptic fragment from pancreatic islets have been detected at high frequency in the sera of patients with insulin-dependent diabetes mellitus (IDDM). IA-2, a newly isolated transmembrane protein tyrosine phosphatase, is a major islet cell autoantigen in IDDM and the precursor of a 40-kD tryptic fragment. To express large quantities of recombinant IA-2 protein and analyse post-translational modifications we expressed full-length human IA-2 in baculovirus-infected Sf-9 cells. IA-2 expression was analysed by Western blot and by immunoprecipitation of 35S-methionine-radiolabelled proteins with rabbit antisera or IDDM sera. A 120-kD IA-2 protein was detected during the early, but not the late, phase of the infection. Pulse-chase experiments showed that the 120-kD protein was processed into fragments of 64 kD and smaller fragments of approximately 50 kD, 38 kD and 32 kD. The 64-kD fragment appeared as a doublet. Tunicamycin and PNGase F treatment down-shifted the 120-kD protein and the 64-kD doublet into lower molecular weight bands, suggesting that both were glycosylated. Trypsin treatment converted the 120-kD protein and the 64-kD doublet into a 40-kD fragment. Baculovirus-expressed IA-2 was as sensitive or slightly more sensitive than in vitro translated IA-2 in detecting autoantibodies to IA-2: 66% of sera from newly diagnosed IDDM patients reacted with baculovirus-expressed IA-2 compared with 59% of the same sera which reacted with in vitro translated IA-2. It is concluded that baculovirus-expressed IA-2 is a good source of autoantigen and that a number of lower molecular weight fragments with which IDDM autoantibodies react are derived from the 120-kD full-length IA-2 molecule.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9737664-1612192,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9737664-1967440,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9737664-2494430,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9737664-3047011,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9737664-3047122,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9737664-3211139,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9737664-3282855,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9737664-4063349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9737664-6206060,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9737664-7045690,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9737664-7568143,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9737664-7571420,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9737664-7594559,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9737664-7657822,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9737664-7926297,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9737664-7980563,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9737664-8024693,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9737664-8144912,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9737664-8166626,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9737664-8200974,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9737664-8325989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9737664-8344280,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9737664-8612696,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9737664-8637868,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9737664-8665506,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9737664-8692821,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9737664-8805677,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9737664-8933284,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9737664-8971079
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0009-9104
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
113
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
367-72
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9737664-Animals,
pubmed-meshheading:9737664-Antigen Presentation,
pubmed-meshheading:9737664-Autoantigens,
pubmed-meshheading:9737664-Diabetes Mellitus, Type 1,
pubmed-meshheading:9737664-Humans,
pubmed-meshheading:9737664-Molecular Weight,
pubmed-meshheading:9737664-Precipitin Tests,
pubmed-meshheading:9737664-Protein Tyrosine Phosphatase, Non-Receptor Type 1,
pubmed-meshheading:9737664-Protein Tyrosine Phosphatases,
pubmed-meshheading:9737664-Rabbits,
pubmed-meshheading:9737664-Recombinant Proteins,
pubmed-meshheading:9737664-Spodoptera,
pubmed-meshheading:9737664-Trypsin
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pubmed:year |
1998
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pubmed:articleTitle |
Expression, characterization, processing and immunogenicity of an insulin-dependent diabetes mellitus autoantigen, IA-2, in Sf-9 cells.
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pubmed:affiliation |
Experimental Medicine Section, Oral Infection and Immunity Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, MD, USA.
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pubmed:publicationType |
Journal Article
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