Linked Life Data

9736770

Source:http://linkedlifedata.com/resource/pubmed/id/9736770

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1998-12-1
pubmed:databankReference
pubmed:abstractText
Target genes for the helicase-like transcription factor (HLTF), a member of the SNF/SWI family, were immunoprecipitated from HeLa chromatin fragments with an anti-HLTF antibody. A 182 bp fragment ( HEFT1 ) presented 87% sequence identity with 3.3 kb dispersed repeats from the 4q35 D4Z4 locus linked to facioscapulohumeral muscular dystrophy (FSHD). The HEFT1 loci were, however, not genetically linked to FSHD. Transfection and in vitro binding studies identified within HEFT1 a promoter whose basal activity required a GC box activated by Sp1 or Sp3. A 4.4 kb homologous transcript was found mostly in human skeletal muscle and heart. A 1.2 kb cDNA fragment was cloned that encoded a 170 amino acid protein (DUX1) with two paired-type homeodomains. In vitro translated DUX1 specifically interacted in electrophoretic mobility shift assay (EMSA) with a P5 oligonucleotide (5'-GATCTGAGTCTAATTGAGAATTACTGTAC-3'). DUX1 co-expression activated up to 5-fold transient expression in insect cells of a minimal promoter-luciferase construct fused to P5. The presence of 20 kDa DUX1 in vivo in rhabdomyosarcoma TE671 cell extracts was shown by western blotting with a rabbit antiserum raised against a DUX1 peptide. This antiserum suppressed a TE671 protein-P5 complex in EMSA with identical migration as the in vitro translated DUX1-P5 complex. Genomic PCR experiments could not identify a gene fragment linking the HEFT1 and DUX1 sequences, which present one mismatch in their overlapping region. However, a similar gene was found in another 3.3 kb element comprising the HEFT1 promoter and a DUX1 -like open reading frame. In addition, homologous gene sequences were identified in 3.3 kb elements of the D4Z4/FSHD locus, considered until now 'junk' DNA.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0964-6906
pubmed:author
pubmed:issnType
Print
pubmed:volume
7
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1681-94
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:9736770-Amino Acid Sequence, pubmed-meshheading:9736770-Animals, pubmed-meshheading:9736770-Antibodies, Monoclonal, pubmed-meshheading:9736770-Base Sequence, pubmed-meshheading:9736770-Chromosome Mapping, pubmed-meshheading:9736770-Cloning, Molecular, pubmed-meshheading:9736770-DNA-Binding Proteins, pubmed-meshheading:9736770-Homeodomain Proteins, pubmed-meshheading:9736770-Humans, pubmed-meshheading:9736770-Interspersed Repetitive Sequences, pubmed-meshheading:9736770-Molecular Sequence Data, pubmed-meshheading:9736770-Muscular Dystrophies, pubmed-meshheading:9736770-Open Reading Frames, pubmed-meshheading:9736770-Precipitin Tests, pubmed-meshheading:9736770-Promoter Regions, Genetic, pubmed-meshheading:9736770-Rabbits, pubmed-meshheading:9736770-Sp1 Transcription Factor, pubmed-meshheading:9736770-Sp3 Transcription Factor, pubmed-meshheading:9736770-TATA Box, pubmed-meshheading:9736770-Trans-Activators, pubmed-meshheading:9736770-Transcription Factors
pubmed:year
1998
pubmed:articleTitle
Characterization of a double homeodomain protein (DUX1) encoded by a cDNA homologous to 3.3 kb dispersed repeated elements.
pubmed:affiliation
Centre for Molecular and Vascular Biology and Centre for Human Genetics, University of Leuven, Herestraat 49, B-3000 Leuven, Belgium.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't