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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
19
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pubmed:dateCreated |
1998-10-26
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pubmed:abstractText |
Serotonin (5-hydroxytryptamine) type 3 receptors (5-HT3R) and nicotinic acetylcholine receptors are structurally and functionally related proteins, yet distinct members of the family of ligand-gated ion channels. For most members of this family a diversity of heteromeric receptors is known at present. In contrast, known 5-HT3R subunits are all homologs of the same 5-HT3R-A subunit and form homopentameric receptors. Here we show, by heterologous expression followed by immunoprecipitation, that 5-HT3R and nicotinic acetylcholine receptor alpha4 subunits coassemble into a novel type of heteromeric ligand-gated ion channel, which is activated by 5-HT. The Ca2+ permeability of this heteromeric ion channel is enhanced as compared with that of the homomeric 5-HT3R channel. Heteromeric 5-HT3/alpha4 and homomeric 5-HT3Rs have similar pharmacological profiles, but distinct sensitivities to block by the antagonist d-tubocurarine. Coassembly of subunits beyond the boundaries of ligand-gated ion channel families may constitute an important mechanism contributing to the diverse properties and functions of native neurotransmitter receptors.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9736758-1718042,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9736758-2286832,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9736758-2600815,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9736758-3273409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9736758-3467376,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9736758-6313909,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9736758-7520482,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9736758-7539990,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9736758-7565620,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9736758-7620711,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9736758-7683998,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9736758-7808432,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9736758-7891159,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9736758-7984286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9736758-8011338,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9736758-8229003,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9736758-8247158,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9736758-8330206,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9736758-8461135,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9736758-8581403,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9736758-8662278,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9736758-8764583,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9736758-8848005,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9736758-8851499,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9736758-9006978,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9736758-9023878,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9736758-9098692,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9736758-9282959,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9736758-9334409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9736758-9356400,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9736758-9401968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9736758-9489730,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9736758-9681938
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channels,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Fura-2,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Nicotinic,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Serotonin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Serotonin, 5-HT3,
http://linkedlifedata.com/resource/pubmed/chemical/Serotonin,
http://linkedlifedata.com/resource/pubmed/chemical/Tubocurarine
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
95
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
11456-61
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pubmed:dateRevised |
2010-8-25
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pubmed:meshHeading |
pubmed-meshheading:9736758-Animals,
pubmed-meshheading:9736758-Calcium,
pubmed-meshheading:9736758-Calcium Channels,
pubmed-meshheading:9736758-Cell Line,
pubmed-meshheading:9736758-DNA, Complementary,
pubmed-meshheading:9736758-Electrophysiology,
pubmed-meshheading:9736758-Fura-2,
pubmed-meshheading:9736758-Gene Expression,
pubmed-meshheading:9736758-Humans,
pubmed-meshheading:9736758-Microinjections,
pubmed-meshheading:9736758-Oocytes,
pubmed-meshheading:9736758-Precipitin Tests,
pubmed-meshheading:9736758-Protein Conformation,
pubmed-meshheading:9736758-Receptors, Nicotinic,
pubmed-meshheading:9736758-Receptors, Serotonin,
pubmed-meshheading:9736758-Receptors, Serotonin, 5-HT3,
pubmed-meshheading:9736758-Serotonin,
pubmed-meshheading:9736758-Transfection,
pubmed-meshheading:9736758-Tubocurarine,
pubmed-meshheading:9736758-Xenopus
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pubmed:year |
1998
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pubmed:articleTitle |
Promiscuous coassembly of serotonin 5-HT3 and nicotinic alpha4 receptor subunits into Ca(2+)-permeable ion channels.
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pubmed:affiliation |
Research Institute of Toxicology, Utrecht University, P.O. Box 80. 176, NL-3508 TD Utrecht, The Netherlands.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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