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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
18
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pubmed:dateCreated |
1998-10-2
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pubmed:abstractText |
Previous pharmacological studies have indicated the possible existence of functional interactions between mu-, delta- and kappa-opioid receptors in the CNS. We have investigated this issue using a genetic approach. Here we describe in vitro and in vivo functional activity of delta- and kappa-opioid receptors in mice lacking the mu-opioid receptor (MOR). Measurements of agonist-induced [35S]GTPgammaS binding and adenylyl cyclase inhibition showed that functional coupling of delta- and kappa-receptors to G-proteins is preserved in the brain of mutant mice. In the mouse vas deferens bioassay, deltorphin II and cyclic[D-penicillamine2, D-penicillamine5] enkephalin exhibited similar potency to inhibit smooth muscle contraction in both wild-type and MOR -/- mice. delta-Analgesia induced by deltorphin II was slightly diminished in mutant mice, when the tail flick test was used. Deltorphin II strongly reduced the respiratory frequency in wild-type mice but not in MOR -/- mice. Analgesic and respiratory responses produced by the selective kappa-agonist U-50,488H were unchanged in MOR-deficient mice. In conclusion, the preservation of delta- and kappa-receptor signaling properties in mice lacking mu-receptors provides no evidence for opioid receptor cross-talk at the cellular level. Intact antinociceptive and respiratory responses to the kappa-agonist further suggest that the kappa-receptor mainly acts independently from the mu-receptor in vivo. Reduced delta-analgesia and the absence of delta-respiratory depression in MOR-deficient mice together indicate that functional interactions may take place between mu-receptors and central delta-receptors in specific neuronal pathways.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/3,4-Dichloro-N-methyl-N-(2-(1-pyrrol...,
http://linkedlifedata.com/resource/pubmed/chemical/Analgesics,
http://linkedlifedata.com/resource/pubmed/chemical/Analgesics, Non-Narcotic,
http://linkedlifedata.com/resource/pubmed/chemical/Analgesics, Opioid,
http://linkedlifedata.com/resource/pubmed/chemical/Anti-Arrhythmia Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Benzofurans,
http://linkedlifedata.com/resource/pubmed/chemical/Enkephalin, Ala(2)-MePhe(4)-Gly(5)-,
http://linkedlifedata.com/resource/pubmed/chemical/Enkephalin, D-Penicillamine (2,5)-,
http://linkedlifedata.com/resource/pubmed/chemical/Enkephalins,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Oligopeptides,
http://linkedlifedata.com/resource/pubmed/chemical/Pyrrolidines,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Opioid,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Opioid, delta,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Opioid, kappa,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Opioid, mu,
http://linkedlifedata.com/resource/pubmed/chemical/deltorphin II, Ala(2)-,
http://linkedlifedata.com/resource/pubmed/chemical/enadoline
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0270-6474
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pubmed:author |
pubmed-author:BoudinotEE,
pubmed-author:Denavit-SaubiéMM,
pubmed-author:FoutzA SAS,
pubmed-author:KiefferB LBL,
pubmed-author:MaldonadoRR,
pubmed-author:MatthesH WHW,
pubmed-author:NegriLL,
pubmed-author:RoquesB PBP,
pubmed-author:SeveriniCC,
pubmed-author:SmadjaCC,
pubmed-author:ValverdeOO,
pubmed-author:VoneschJ LJL
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
18
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7285-95
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9736649-3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benz...,
pubmed-meshheading:9736649-Analgesics,
pubmed-meshheading:9736649-Analgesics, Non-Narcotic,
pubmed-meshheading:9736649-Analgesics, Opioid,
pubmed-meshheading:9736649-Animals,
pubmed-meshheading:9736649-Anti-Arrhythmia Agents,
pubmed-meshheading:9736649-Benzofurans,
pubmed-meshheading:9736649-Enkephalin, Ala(2)-MePhe(4)-Gly(5)-,
pubmed-meshheading:9736649-Enkephalin, D-Penicillamine (2,5)-,
pubmed-meshheading:9736649-Enkephalins,
pubmed-meshheading:9736649-Female,
pubmed-meshheading:9736649-GTP-Binding Proteins,
pubmed-meshheading:9736649-Male,
pubmed-meshheading:9736649-Mice,
pubmed-meshheading:9736649-Mice, Inbred C57BL,
pubmed-meshheading:9736649-Mice, Knockout,
pubmed-meshheading:9736649-Neurons,
pubmed-meshheading:9736649-Oligopeptides,
pubmed-meshheading:9736649-Pain Measurement,
pubmed-meshheading:9736649-Pyrrolidines,
pubmed-meshheading:9736649-Receptors, Opioid,
pubmed-meshheading:9736649-Receptors, Opioid, delta,
pubmed-meshheading:9736649-Receptors, Opioid, kappa,
pubmed-meshheading:9736649-Receptors, Opioid, mu,
pubmed-meshheading:9736649-Respiration,
pubmed-meshheading:9736649-Signal Transduction,
pubmed-meshheading:9736649-Vas Deferens
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pubmed:year |
1998
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pubmed:articleTitle |
Activity of the delta-opioid receptor is partially reduced, whereas activity of the kappa-receptor is maintained in mice lacking the mu-receptor.
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pubmed:affiliation |
Unité Propre de Recherche 9050 Centre National de la Recherche Scientifique, Ecole Supérieure de Biotechnologie de Strasbourg Université Louis Pasteur, F-67400 Illkirch, Strasbourg, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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