Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1998-10-22
pubmed:abstractText
The influence of assay methodology on the measurement of the active free fraction of ceftriaxone in plasma was determined. The free fraction was measured by three methods: agar diffusion bioassay, precipitation of plasma protein with methanol followed by high-performance liquid chromatography (HPLC) of the supernatant, and ultrafiltration of plasma followed by HPLC of the filtrate. In human serum, the free ceftriaxone levels were significantly lower (P = 0.03) when measured on ultrafiltrates compared to the other two methods. This difference disappeared when dolphin serum was studied. After ultrafiltration, human serum was shown, by Scatchard plot analysis, to have two ceftriaxone binding sites. Species differences were also demonstrated. Hence, in humans, determination of free plasma ceftriaxone varies with the assay method employed.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9736545-2228838, http://linkedlifedata.com/resource/pubmed/commentcorrection/9736545-2393258, http://linkedlifedata.com/resource/pubmed/commentcorrection/9736545-2794558, http://linkedlifedata.com/resource/pubmed/commentcorrection/9736545-3424863, http://linkedlifedata.com/resource/pubmed/commentcorrection/9736545-3579254, http://linkedlifedata.com/resource/pubmed/commentcorrection/9736545-3824437, http://linkedlifedata.com/resource/pubmed/commentcorrection/9736545-3948454, http://linkedlifedata.com/resource/pubmed/commentcorrection/9736545-4028870, http://linkedlifedata.com/resource/pubmed/commentcorrection/9736545-657874, http://linkedlifedata.com/resource/pubmed/commentcorrection/9736545-7053242, http://linkedlifedata.com/resource/pubmed/commentcorrection/9736545-8431018
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0066-4804
pubmed:author
pubmed:issnType
Print
pubmed:volume
42
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2259-61
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Influence of assay methodology on the measurement of free serum ceftriaxone concentrations.
pubmed:affiliation
Earle A. Chiles Research Institute, Providence Portland Medical Center, Portland, Oregon, USA. sue_kohlhepp@phsor.org
pubmed:publicationType
Journal Article