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rdf:type |
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lifeskim:mentions |
umls-concept:C0013879,
umls-concept:C0035696,
umls-concept:C0035820,
umls-concept:C0040421,
umls-concept:C0041904,
umls-concept:C0086418,
umls-concept:C0086860,
umls-concept:C0162493,
umls-concept:C0205374,
umls-concept:C0806987,
umls-concept:C1167622,
umls-concept:C1367714
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pubmed:issue |
4
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pubmed:dateCreated |
1998-9-29
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pubmed:abstractText |
Stimulation of human tonsillar mononuclear cells with interleukin-4 (IL-4) and interferon-gamma (IFN-gamma) rapidly induced the activation of distinct nuclear factors with different mobilities, both of which bind the IL-4 response element (IL-4RE) of CD23b promoter as examined by electrophoretic mobility shift assays (EMSA). Co-treatment of IL-4 and IFN-gamma induced, in addition to the two distinct complexes, a new complex with an intermediate mobility. The IL-4-induced complex reacted with anti-STAT (signal transducers and activators of transcription) 6, resulting in a supershift whereas the formation of the IFN-gamma-induced complex was inhibited by anti-STAT 1. The intermediate complex appeared to react with both anti-STAT 6 and anti-STAT 1. Although IFN-gamma alone did not induce CD23 mRNA transcription, Northern blot analysis revealed a transient up-regulation of the IL-4-induced CD23 mRNA by IFN-gamma within 2 h of IFN-gamma treatment in these tonsillar cells. The results suggest that the IL-4RE of the IL-4-inducible gene can accommodate both IL-4- and IFN-gamma-activated factors, such as STAT 6 and STAT 1, either in homodimeric or heterodimeric forms and the binding of these different proteins to the respective promoter may play a potential regulatory role in the IL-4-inducible gene expression.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Constant Regions,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin E,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Heavy Chains,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, IgE,
http://linkedlifedata.com/resource/pubmed/chemical/STAT1 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/STAT1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Stat1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0161-5890
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
35
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
239-47
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:9736340-Animals,
pubmed-meshheading:9736340-Antibodies,
pubmed-meshheading:9736340-DNA-Binding Proteins,
pubmed-meshheading:9736340-Humans,
pubmed-meshheading:9736340-Immunoglobulin Constant Regions,
pubmed-meshheading:9736340-Immunoglobulin E,
pubmed-meshheading:9736340-Immunoglobulin Heavy Chains,
pubmed-meshheading:9736340-Interferon-gamma,
pubmed-meshheading:9736340-Interleukin-4,
pubmed-meshheading:9736340-Leukocytes, Mononuclear,
pubmed-meshheading:9736340-Mice,
pubmed-meshheading:9736340-Mice, Knockout,
pubmed-meshheading:9736340-Palatine Tonsil,
pubmed-meshheading:9736340-Promoter Regions, Genetic,
pubmed-meshheading:9736340-RNA, Messenger,
pubmed-meshheading:9736340-Receptors, IgE,
pubmed-meshheading:9736340-STAT1 Transcription Factor,
pubmed-meshheading:9736340-Trans-Activators,
pubmed-meshheading:9736340-Up-Regulation
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pubmed:year |
1998
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pubmed:articleTitle |
Interferon-gamma-induced factor binding to the interleukin-4-responsive element of CD23b promoter in human tonsillar mononuclear cells: role in transient up-regulation of the interleukin-4-induced CD23b mRNA.
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pubmed:affiliation |
Department of Biology and Institute of Basic Science, College of Natural Science, Sung Kyun Kwan Univ. Suwon, Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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