|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
1978-9-25
|
|
pubmed:abstractText |
Cells of the 315LV-1 (derived from NP1) variant line of MOPC 315 contain approximately 1% the normal intracellular level of the heavy (alpha) chain of IgA and no detectable light (lambda2) chain. The synthesis rate of alpha-chain in the variant, however, is similar to that in cells of the parent line. Moreover the relative amount of translatable alpha-chain mRNA that can be extracted from 315LV-1 cells is about the same as for parental cells. No light-chain synthesis can be detected either in vivo or in vitro in a wheat germ cell-free system. The 315LV-1 heavy chain synthesized in vivo or in vitro has slightly greater electrophoretic mobility than normal H chain and turns over rapidly intracellularly. The variant fails to secrete any of its heavy chain, despite the fact that its H chain mRNA is bound to membranes, as one would expect for a secretory protein message. Fusion of 315LV-1 cells with cells of a kappa-producing MPC 11 variant line leads to stabilization of the intracellular H chain and also to full recovery of secretion of the H chain as an H2L2 molecule.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/97359-1061088,
http://linkedlifedata.com/resource/pubmed/commentcorrection/97359-1175627,
http://linkedlifedata.com/resource/pubmed/commentcorrection/97359-1252084,
http://linkedlifedata.com/resource/pubmed/commentcorrection/97359-16591486,
http://linkedlifedata.com/resource/pubmed/commentcorrection/97359-325006,
http://linkedlifedata.com/resource/pubmed/commentcorrection/97359-403522,
http://linkedlifedata.com/resource/pubmed/commentcorrection/97359-4113870,
http://linkedlifedata.com/resource/pubmed/commentcorrection/97359-4141694,
http://linkedlifedata.com/resource/pubmed/commentcorrection/97359-414225,
http://linkedlifedata.com/resource/pubmed/commentcorrection/97359-4171435,
http://linkedlifedata.com/resource/pubmed/commentcorrection/97359-4180613,
http://linkedlifedata.com/resource/pubmed/commentcorrection/97359-4205351,
http://linkedlifedata.com/resource/pubmed/commentcorrection/97359-4205352,
http://linkedlifedata.com/resource/pubmed/commentcorrection/97359-4510289,
http://linkedlifedata.com/resource/pubmed/commentcorrection/97359-4525168,
http://linkedlifedata.com/resource/pubmed/commentcorrection/97359-4536742,
http://linkedlifedata.com/resource/pubmed/commentcorrection/97359-4850204,
http://linkedlifedata.com/resource/pubmed/commentcorrection/97359-5010390,
http://linkedlifedata.com/resource/pubmed/commentcorrection/97359-5263020,
http://linkedlifedata.com/resource/pubmed/commentcorrection/97359-5273448,
http://linkedlifedata.com/resource/pubmed/commentcorrection/97359-605383,
http://linkedlifedata.com/resource/pubmed/commentcorrection/97359-804239,
http://linkedlifedata.com/resource/pubmed/commentcorrection/97359-804509,
http://linkedlifedata.com/resource/pubmed/commentcorrection/97359-809509,
http://linkedlifedata.com/resource/pubmed/commentcorrection/97359-811671,
http://linkedlifedata.com/resource/pubmed/commentcorrection/97359-821618,
http://linkedlifedata.com/resource/pubmed/commentcorrection/97359-825374,
http://linkedlifedata.com/resource/pubmed/commentcorrection/97359-999824
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
AIM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jul
|
|
pubmed:issn |
0022-1007
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
1
|
|
pubmed:volume |
148
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
301-12
|
|
pubmed:dateRevised |
2010-9-1
|
|
pubmed:meshHeading |
pubmed-meshheading:97359-Animals,
pubmed-meshheading:97359-Cell Line,
pubmed-meshheading:97359-Immunoglobulin Heavy Chains,
pubmed-meshheading:97359-Immunoglobulin alpha-Chains,
pubmed-meshheading:97359-Immunoglobulin lambda-Chains,
pubmed-meshheading:97359-Mice,
pubmed-meshheading:97359-Mice, Inbred BALB C,
pubmed-meshheading:97359-Plasmacytoma,
pubmed-meshheading:97359-RNA, Messenger,
pubmed-meshheading:97359-RNA, Neoplasm
|
|
pubmed:year |
1978
|
|
pubmed:articleTitle |
Control of immunoglobulin secretion in the murine plasmacytoma line MOPC 315.
|
|
pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.
|
