|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
10
|
|
pubmed:dateCreated |
1998-10-7
|
|
pubmed:abstractText |
The induction of an efficient CD4(+) T-cell response against hepatitis C virus (HCV) is critical for control of the chronicity of HCV infection. The ability of HCV structural protein endogenously expressed in an antigen-presenting cell (APC) to be presented by class II major histocompatibility complex molecules to CD4(+) T cells was investigated by in vitro culture analyses using HCV core-specific T-cell lines and autologous Epstein-Barr virus-transformed B-lymphoblastoid cell lines (B-LCLs) expressing structural HCV antigens. The T- and B-cell lines were generated from peripheral blood mononuclear cells derived from HCV-infected patients. Expression and intracellular localization of core protein in transfected cells were determined by immunoblotting and immunofluorescence. By stimulation with autologous B-LCLs expressing viral antigens, strong T-cell proliferative responses were induced in two of three patients, while no substantial stimulatory effects were produced by B-LCLs expressing a control protein (chloramphenicol acetyltransferase) or by B-LCLs alone. The results showed that transfected B cells presented mainly endogenously synthesized core peptides. Presentation of secreted antigens from adjacent antigen-expressing cells was not enough to stimulate a core-specific T-cell response. Only weak T-cell proliferative responses were generated by stimulation with B-LCLs that had been pulsed beforehand with at least a 10-fold-higher amount of transfected COS cells in the form of cell lysate, suggesting that presentation of antigens released from dead cells in the B-LCL cultures had a minimal role. Titrating numbers of APCs, we showed that as few as 10(4) transfected B-LCL APCs were sufficient to stimulate T cells. This presentation pathway was found to be leupeptin sensitive, and it can be blocked by antibody to HLA class II (DR). In addition, expression of a costimulatory signal by B7/BB1 on B cells was essential for T-cell activation.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-1336977,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-1372018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-1648221,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-1650475,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-1695549,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-1709694,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-1847440,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-1847722,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-1891045,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-1910679,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-1959884,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-2113314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-2170265,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-2371288,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-2404209,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-2411815,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-2456369,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-2509915,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-2523562,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-2543701,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-2794510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-2961215,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-3034602,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-3036075,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-3257576,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-3538822,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-3934268,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-4192904,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-6203977,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-6242349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-6274961,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-7475549,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-7498659,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-7512163,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-7525839,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-7529278,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-7533182,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-7541426,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-7570000,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-7597443,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-7664784,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-7912996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-7930486,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-8011301,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-8090737,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-8172137,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-8294086,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-8425701,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-8633023,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-8643693,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-8698862,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-8700831,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-8760802,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-8823309,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-8855177,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-92183,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733874-9641645
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Oct
|
|
pubmed:issn |
0022-538X
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
72
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
8301-8
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
|
