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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1998-10-7
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pubmed:abstractText |
The RNA elements that are required for replication of defective interfering (DI) RNA of the JHM strain of mouse hepatitis virus (MHV) consist of three discontinuous genomic regions: about 0.46 to 0.47 kb from both terminal sequences and an internal 58-nucleotide (nt)-long sequence (58-nt region) present at about 0.9 kb from the 5' end of the DI genome. The internal region is important for positive-strand DI RNA synthesis (Y. N. Kim and S. Makino, J. Virol. 69:4963-4971, 1995). We further characterized the 58-nt region in the present study and obtained the following results. (i) The positive-strand RNA structure in solution was comparable with that predicted by computer modeling. (ii) Positive-strand RNA secondary structure, but not negative-strand RNA structure, was important for the biological function of the region. (iii) The biological function had a sequence-specific requirement. We discuss possible mechanisms by which the internal cis-acting signal drives MHV positive-strand DI RNA synthesis.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733830-1326650,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733830-1383562,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733830-1656085,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733830-1846489,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733830-2033672,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733830-207885,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733830-2468070,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733830-2545027,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733830-2555555,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733830-2845661,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733830-2922271,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733830-2985802,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733830-4365902,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733830-6163133,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733830-6169842,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733830-6802981,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733830-7609066,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733830-7884846,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733830-7966301,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733830-8039508,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733830-8212595,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733830-8230443,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733830-8254744,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733830-8291254,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733830-8345521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733830-8388482,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733830-8396672,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733830-8525608,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733830-8614984,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733830-8709192,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733830-9275159
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
72
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7926-33
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9733830-Animals,
pubmed-meshheading:9733830-Base Sequence,
pubmed-meshheading:9733830-Cell Line,
pubmed-meshheading:9733830-Mice,
pubmed-meshheading:9733830-Molecular Sequence Data,
pubmed-meshheading:9733830-Murine hepatitis virus,
pubmed-meshheading:9733830-Mutagenesis, Site-Directed,
pubmed-meshheading:9733830-Nucleic Acid Conformation,
pubmed-meshheading:9733830-RNA, Viral,
pubmed-meshheading:9733830-Solutions
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pubmed:year |
1998
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pubmed:articleTitle |
Importance of the positive-strand RNA secondary structure of a murine coronavirus defective interfering RNA internal replication signal in positive-strand RNA synthesis.
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pubmed:affiliation |
Department of Microbiology, The University of Texas at Austin, Austin, Texas 78712, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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