pubmed-article:9733524 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:9733524 | lifeskim:mentions | umls-concept:C1095831 | lld:lifeskim |
pubmed-article:9733524 | lifeskim:mentions | umls-concept:C0004755 | lld:lifeskim |
pubmed-article:9733524 | lifeskim:mentions | umls-concept:C0014442 | lld:lifeskim |
pubmed-article:9733524 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
pubmed-article:9733524 | lifeskim:mentions | umls-concept:C1171362 | lld:lifeskim |
pubmed-article:9733524 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
pubmed-article:9733524 | lifeskim:mentions | umls-concept:C1515670 | lld:lifeskim |
pubmed-article:9733524 | lifeskim:mentions | umls-concept:C0679058 | lld:lifeskim |
pubmed-article:9733524 | lifeskim:mentions | umls-concept:C1547699 | lld:lifeskim |
pubmed-article:9733524 | lifeskim:mentions | umls-concept:C2700640 | lld:lifeskim |
pubmed-article:9733524 | pubmed:issue | 1 | lld:pubmed |
pubmed-article:9733524 | pubmed:dateCreated | 1998-11-18 | lld:pubmed |
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pubmed-article:9733524 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:9733524 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:9733524 | pubmed:abstractText | The sbeIIa and sbeIIb genes, encoding starch-branching enzyme (SBE) IIa and SBEIIb in barley (Hordeum vulgare L.), have been isolated. The 5' portions of the two genes are strongly divergent, primarily due to the 2064-nucleotide-long intron 2 in sbeIIb. The sequence of this intron shows that it contains a retro-transposon-like element. Expression of sbeIIb but not sbeIIa was found to be endosperm specific. The temporal expression patterns for sbeIIa and sbeIIb were similar and peaked around 12 d after pollination. DNA gel-blot analysis demonstrated that sbeIIa and sbeIIb are both single-copy genes in the barley genome. By fluorescence in situ hybridization, the sbeIIa and sbeIIb genes were mapped to chromosomes 2 and 5, respectively. The cDNA clones for SBEIIa and SBEIIb were isolated and sequenced. The amino acid sequences of SBEIIa and SBEIIb were almost 80% identical. The major structural difference between the two enzymes was the presence of a 94-amino acid N-terminal extension in the SBEIIb precursor. The (beta/alpha)8-barrel topology of the alpha-amylase superfamily and the catalytic residues implicated in branching enzymes are conserved in both barley enzymes. | lld:pubmed |
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pubmed-article:9733524 | pubmed:language | eng | lld:pubmed |
pubmed-article:9733524 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:9733524 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:9733524 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:9733524 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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pubmed-article:9733524 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:9733524 | pubmed:month | Sep | lld:pubmed |
pubmed-article:9733524 | pubmed:issn | 0032-0889 | lld:pubmed |
pubmed-article:9733524 | pubmed:author | pubmed-author:SunCC | lld:pubmed |
pubmed-article:9733524 | pubmed:author | pubmed-author:JanssonCC | lld:pubmed |
pubmed-article:9733524 | pubmed:author | pubmed-author:SathishPP | lld:pubmed |
pubmed-article:9733524 | pubmed:author | pubmed-author:AhlandsbergSS | lld:pubmed |
pubmed-article:9733524 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:9733524 | pubmed:volume | 118 | lld:pubmed |
pubmed-article:9733524 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:9733524 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:9733524 | pubmed:pagination | 37-49 | lld:pubmed |
pubmed-article:9733524 | pubmed:dateRevised | 2010-9-10 | lld:pubmed |
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pubmed-article:9733524 | pubmed:year | 1998 | lld:pubmed |
pubmed-article:9733524 | pubmed:articleTitle | The two genes encoding starch-branching enzymes IIa and IIb are differentially expressed in barley. | lld:pubmed |
pubmed-article:9733524 | pubmed:affiliation | Department of Biochemistry, The Arrhenius Laboratories, Stockholm University, S-106 91 Stockholm, Sweden. | lld:pubmed |
pubmed-article:9733524 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:9733524 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |