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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1998-11-18
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pubmed:databankReference |
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pubmed:abstractText |
The sbeIIa and sbeIIb genes, encoding starch-branching enzyme (SBE) IIa and SBEIIb in barley (Hordeum vulgare L.), have been isolated. The 5' portions of the two genes are strongly divergent, primarily due to the 2064-nucleotide-long intron 2 in sbeIIb. The sequence of this intron shows that it contains a retro-transposon-like element. Expression of sbeIIb but not sbeIIa was found to be endosperm specific. The temporal expression patterns for sbeIIa and sbeIIb were similar and peaked around 12 d after pollination. DNA gel-blot analysis demonstrated that sbeIIa and sbeIIb are both single-copy genes in the barley genome. By fluorescence in situ hybridization, the sbeIIa and sbeIIb genes were mapped to chromosomes 2 and 5, respectively. The cDNA clones for SBEIIa and SBEIIb were isolated and sequenced. The amino acid sequences of SBEIIa and SBEIIb were almost 80% identical. The major structural difference between the two enzymes was the presence of a 94-amino acid N-terminal extension in the SBEIIb precursor. The (beta/alpha)8-barrel topology of the alpha-amylase superfamily and the catalytic residues implicated in branching enzymes are conserved in both barley enzymes.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733524-1281436,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733524-1302049,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733524-1440872,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733524-16668750,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733524-1720313,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733524-1745241,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733524-2153053,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733524-2311769,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733524-2441623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733524-2461560,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733524-2828168,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733524-7640529,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733524-7729540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733524-7894509,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733524-7920707,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733524-7991537,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733524-8054988,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733524-8111031,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733524-8136030,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733524-8220472,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733524-8278524,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733524-8281041,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733524-8360192,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733524-8455548,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733524-8455560,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733524-8479422,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733524-8616246,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733524-8704131,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733524-8754683,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733524-8756717,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733524-8870497,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733524-8885169,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733524-8954164,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733524-9008395,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9733524-9159942
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0032-0889
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
118
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
37-49
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pubmed:dateRevised |
2010-9-10
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pubmed:meshHeading |
pubmed-meshheading:9733524-1,4-alpha-Glucan Branching Enzyme,
pubmed-meshheading:9733524-Amino Acid Sequence,
pubmed-meshheading:9733524-Base Sequence,
pubmed-meshheading:9733524-Chromosome Mapping,
pubmed-meshheading:9733524-Cloning, Molecular,
pubmed-meshheading:9733524-DNA, Complementary,
pubmed-meshheading:9733524-DNA, Plant,
pubmed-meshheading:9733524-DNA Primers,
pubmed-meshheading:9733524-Gene Expression Regulation, Developmental,
pubmed-meshheading:9733524-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:9733524-Gene Expression Regulation, Plant,
pubmed-meshheading:9733524-Genes, Plant,
pubmed-meshheading:9733524-Hordeum,
pubmed-meshheading:9733524-In Situ Hybridization, Fluorescence,
pubmed-meshheading:9733524-Introns,
pubmed-meshheading:9733524-Isoenzymes,
pubmed-meshheading:9733524-Molecular Sequence Data,
pubmed-meshheading:9733524-Phylogeny,
pubmed-meshheading:9733524-Sequence Homology, Amino Acid,
pubmed-meshheading:9733524-Sequence Homology, Nucleic Acid
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pubmed:year |
1998
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pubmed:articleTitle |
The two genes encoding starch-branching enzymes IIa and IIb are differentially expressed in barley.
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pubmed:affiliation |
Department of Biochemistry, The Arrhenius Laboratories, Stockholm University, S-106 91 Stockholm, Sweden.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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