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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1998-11-24
|
| pubmed:abstractText |
To investigate the growth-regulating action of estrogen on vascular smooth muscle cells (SMC), effects of beta-17-estradiol (beta-E2) on phenotypic modulation and proliferation of rabbit aortic SMC were observed in vitro. At 10(-8)M, beta-E2 significantly slowed the decrease in volume fraction of myofilaments (Vv myo) of freshly dispersed SMCs in primary culture, indicating an inhibitory effect of beta-E2 on spontaneous phenotypic modulation of SMC from a contractile to a synthetic phenotype. Freshly dispersed SMCs treated with beta-E2 also had a relatively longer quiescent phase than control cells before intense proliferation occurred. This was in contrast to SMCs in passage 2 3 (synthetic state), where beta-E2-treated cells replicated significantly faster than untreated cells. beta-E2 also markedly enhanced the serum-induced DNA synthesis of synthetic SMCs in a concentration-dependent manner within physiological range (10(-10)to 10(-8)M). These findings indicate that the growth-regulating effect of estrogen on vascular SMC is dependent on the cell's phenotypic state. It delays the cell cycle re-entry of the contractile SMCs by retarding their phenotypic modulation: however, once cells have modulated to the synthetic phenotype, it promotes their replication.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0021-9150
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
140
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
97-104
|
| pubmed:dateRevised |
2003-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:9733220-Animals,
pubmed-meshheading:9733220-Cell Cycle,
pubmed-meshheading:9733220-Cell Division,
pubmed-meshheading:9733220-DNA Replication,
pubmed-meshheading:9733220-Estradiol,
pubmed-meshheading:9733220-Female,
pubmed-meshheading:9733220-Muscle, Smooth, Vascular,
pubmed-meshheading:9733220-Muscle Contraction,
pubmed-meshheading:9733220-Phenotype,
pubmed-meshheading:9733220-Rabbits,
pubmed-meshheading:9733220-Thymidine
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
Effect of estrogen on vascular smooth muscle cells is dependent upon cellular phenotype.
|
| pubmed:affiliation |
Centre for Research in Vascular Biology, Department of Anatomical Sciences, University of Queensland, Brisbane, Australia.
|
| pubmed:publicationType |
Journal Article
|