Linked Life Data

9732752

Source:http://linkedlifedata.com/resource/pubmed/id/9732752

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1998-9-24
pubmed:abstractText
We isolated interspecific hybrids between normal human leukocytes and a Chinese hamster ovary cell line that has mutations in three genes, leuS, emtB, and chr, all of which are linked to chromosome 2. The conditionally lethal mutation in the leuS gene in this cell line affects leucyl-tRNA synthetase and renders the cell line nonviable at 39 degrees C. The mutation in the emtB locus alters ribosomal protein S14 and results in the cell line being resistant to the protein synthesis inhibitor, emetine, while the mutation in the chr locus renders the cells resistant to sodium chromate. The interspecific hybrids were selected at 39 degrees C so that they were required to retain and express the human leuS gene. Ten out of ten such heat-resistant hybrids also expressed the human emtB and chr genes. Segregants selected as having lost the human emtB gene simultaneously lost the human chr and leuS genes as well. The linkage relationship between these three genes has thus been conserved during the evolution of the human and Chinese hamster genomes. All three genes were localized to human chromosome 5. Furthermore, our results indicate that the ribosomal protein product of the human emtB gene is incorporated into functional ribosomes in place of the human corresponding Chinese hamster protein, raising several interesting questions concerning the coordinate regulation of genes encoding ribosomal proteins in mammalian cells.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0098-0366
pubmed:author
pubmed:issnType
Print
pubmed:volume
8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
245-64
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Linkage of the leuS, emtB, and chr genes on chromosome 5 in humans and expression of human genes encoding protein synthetic components in human--Chinese hamster hybrids.
pubmed:affiliation
Department of Biological Chemistry, Calfornia College of Medicine, University of California, Irvine 92717, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't