Linked Life Data

9728813

Source:http://linkedlifedata.com/resource/pubmed/id/9728813

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1998-11-19
pubmed:abstractText
The ability of bovine blastocysts to recover after cryopreservation and thawing procedures is often assessed by evaluating their re-expansion during in vitro co-culture. However, the influence of factors such as feeder cell type and gas atmosphere on blastocyst survival and evolution have never been considered. This study therefore compared two cell co-culture systems and two different gas atmospheres to assess survival of in vitro produced bovine blastocysts after vitrification. Day-7 blastocysts (n = 181) were vitrified in a mixture of 25% glycerol/25% ethylene glycol. After warming and dilution, they were co-cultured either on Buffalo rat liver cells (BRL CC cell line) or on granulosa cells (GR CC primary culture) in TCM 199 supplemented with 10% FCS and under an atmosphere of 5% or 20% O2. Surviving and hatching rates were recorded at 24 h intervals for 3 days. After 72 h of culture, surviving blastocysts were treated for differential counting of inner cell mass (ICM) and trophectoderm cells. Blastocyst survival rates were higher when BRL and granulosa co-culture were performed under 20% oxygen as compared to 5% oxygen (20% O2: 62% vs. 5% O2: 25%, P < 0.0001). However, the quality of blastocysts surviving in the granulosa co-culture condition was lower under 20% O2 than under 5% O2 as indicated by lower total and trophectoderm cell numbers (respectively 79 +/- 6 and 56 +/- 6 at 20% O2 vs. 100 +/- 10 and 74 +/- 10 at 5% O2, P < 0.05), by an altered ICM/trophectoderm ratio (20% O2: 28% vs. 5% O2: 23%, P < 0.05), by a higher total nuclear fragmentation (20% O2: 3.7% vs. 5% O2: 1.5%, P < 0.05) and a trend to decreased hatching (20% O2: 32% vs. 5% O2: 81%, P = 0.07). Whereas, for BRL co-culture, 20% O2 yielded higher quality blastocysts than 5% O2 as evaluated by higher ICM and trophectoderm cell numbers (19 +/- 1 and 71 +/- 5 at 20% O2 vs. 15 +/- 2 and 48 +/- 9 at 5% O2, respectively, P < 0.05), by lower nuclear fragmentation in the ICM (20% O2: 2.2% vs. 5% O2: 6.7%, P < 0.05). In conclusion, co-culture conditions may influence blastocysts survival and quality after cryopreservation. In our conditions, co-culture with BRL cells under 20% O2 seems to be the best combination to evaluate blastocyst survival and quality after vitrification.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0378-4320
pubmed:author
pubmed:issnType
Print
pubmed:day
30
pubmed:volume
52
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
39-50
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:9728813-Animals, pubmed-meshheading:9728813-Bisbenzimidazole, pubmed-meshheading:9728813-Blastocyst, pubmed-meshheading:9728813-Cattle, pubmed-meshheading:9728813-Cell Line, pubmed-meshheading:9728813-Coculture Techniques, pubmed-meshheading:9728813-Cryopreservation, pubmed-meshheading:9728813-Cryoprotective Agents, pubmed-meshheading:9728813-Female, pubmed-meshheading:9728813-Fertilization in Vitro, pubmed-meshheading:9728813-Fluorescent Dyes, pubmed-meshheading:9728813-Granulosa Cells, pubmed-meshheading:9728813-Liver, pubmed-meshheading:9728813-Male, pubmed-meshheading:9728813-Oocytes, pubmed-meshheading:9728813-Oxygen, pubmed-meshheading:9728813-Pregnancy, pubmed-meshheading:9728813-Propidium, pubmed-meshheading:9728813-Rabbits, pubmed-meshheading:9728813-Rats, pubmed-meshheading:9728813-Rats, Inbred BUF, pubmed-meshheading:9728813-Sperm-Ovum Interactions
pubmed:year
1998
pubmed:articleTitle
Comparison of two co-culture systems to assess the survival of in vitro produced bovine blastocysts after vitrification.
pubmed:affiliation
Unité des Sciences Vétérinaires, UCL, Louvain-La-Neuve, Belgium.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't