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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1998-9-10
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pubmed:abstractText |
C-reactive protein (CRP) is a unique serum pentraxin and the prototype acute phase reactant. CRP is a ligand for specific receptors on phagocytic leukocytes, and mediates activation reactions of monocytes/macrophages, but inhibits the respiratory burst of neutrophils (PMN). Since CRP selectively accumulates at inflammatory sites in which IL-8 is also produced, we tested the effects of CRP on the responsiveness of PMN to IL-8 and the bacterial chemotactic peptide, FMLP-phenylalanine (FMLPP). Purified human CRP inhibited the chemotactic response of PMN to IL-8 and FMLPP. A mouse IgM mAb that was generated against the leukocyte CRP receptor (CRP-R) also inhibited the chemotactic response. Incubation of purified CRP with activated PMN generated CRP-derived peptides that also inhibited chemotaxis. A synthetic CRP peptide (residues 27-38) that binds to the CRP-R had weak chemotactic activity, whereas two other CRP synthetic peptides (residues 174-185 and 191-205) inhibited chemotaxis of PMNs to both IL-8 and FMLPP. CRP did not alter receptor-specific binding of IL-8, but exerted its effect at the level of signaling. CRP augmented both IL-8- and FMLPP-induced mitogen-activated protein kinase (extracellular signal-regulated kinase-2) activity. CRP at acute phase levels increased both agonist-induced and noninduced phosphatidylinositol-3 kinase activity. The results suggest a role for CRP as a regulator of leukocyte infiltration at inflammatory sites.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/C-Reactive Protein,
http://linkedlifedata.com/resource/pubmed/chemical/C-reactive protein receptor, human,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Calmodulin-Dependent...,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-8,
http://linkedlifedata.com/resource/pubmed/chemical/N-Formylmethionine...,
http://linkedlifedata.com/resource/pubmed/chemical/N-formyl-methionyl-leucyl-phenylalan...,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol 3-Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-8A
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
161
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2533-40
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:9725253-Amino Acid Sequence,
pubmed-meshheading:9725253-Antibodies, Monoclonal,
pubmed-meshheading:9725253-Antigens, CD,
pubmed-meshheading:9725253-C-Reactive Protein,
pubmed-meshheading:9725253-Calcium-Calmodulin-Dependent Protein Kinases,
pubmed-meshheading:9725253-Chemotaxis, Leukocyte,
pubmed-meshheading:9725253-HL-60 Cells,
pubmed-meshheading:9725253-Humans,
pubmed-meshheading:9725253-Interleukin-8,
pubmed-meshheading:9725253-Molecular Sequence Data,
pubmed-meshheading:9725253-N-Formylmethionine Leucyl-Phenylalanine,
pubmed-meshheading:9725253-Neutrophils,
pubmed-meshheading:9725253-Peptide Fragments,
pubmed-meshheading:9725253-Phosphatidylinositol 3-Kinases,
pubmed-meshheading:9725253-Protein Binding,
pubmed-meshheading:9725253-Receptors, Immunologic,
pubmed-meshheading:9725253-Receptors, Interleukin,
pubmed-meshheading:9725253-Receptors, Interleukin-8A,
pubmed-meshheading:9725253-Signal Transduction
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pubmed:year |
1998
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pubmed:articleTitle |
Effect of human C-reactive protein on chemokine and chemotactic factor-induced neutrophil chemotaxis and signaling.
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pubmed:affiliation |
Department of Microbiology and Comprehensive Cancer Center, Ohio State University, Columbus 43210, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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