Linked Life Data

9724033

Source:http://linkedlifedata.com/resource/pubmed/id/9724033

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1998-9-23
pubmed:databankReference
pubmed:abstractText
The porcine steroidogenic factor-1 gene (pSF-1) was cloned using a combination of genomic and RT-PCR based cloning methods. pSF-1 consists of an open reading frame of 1383 nt corresponding to a deduced amino acid sequence of 461 aa, similar to bovine and human SF-1. Sequence homologies between pSF-1 and human, bovine and mouse molecules indicate strong evolutionary conservation at both the nt and aa levels. Northern analysis of pSF-1 expression in adult steroidogenic tissues correlated with porcine steroidogenic acute regulatory protein gene (pStAR) and porcine side chain cleavage (pP450scc) gene expression. Notably, pSF-1 expression was readily detected in neonatal testes, absent at 3 weeks of age, and again readily detected at 3 months and in adult testes. pSF-1 expression was weak but detectable in placental tissues at various times of gestation, and was correlated with pStAR and pP450scc expression, indicating classical steroidogenesis in this organ. In developing gonads from 6-12 weeks of gestation, i.e. during the time of sex differentiation in the pig, Northern analysis demonstrated increasing expression of PSF-1 in fetal testes and no expression in ovaries. This expression pattern was paralleled for pStAR, pP450scc, and porcine Müllerian inhibitory substance (pMIS), consistent with pSF-1 involvement in both steroid and protein hormone secretions of the developing testes during sex differentiation. Porcine SRY HMG-box related gene-9 (pSOX-9) expression also paralleled that of pSF-1 in developing testes. In contrast, DSS-AHC critical region on the X chromosome, gene 1 (pDAX-1) was expressed predominantly in the developing ovaries, indicating a possible reciprocal regulation of pSF-1 and pDAX-1 genes in developing pig testes and ovaries.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0013-7227
pubmed:author
pubmed:issnType
Print
pubmed:volume
139
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3803-12
pubmed:dateRevised
2009-8-12
pubmed:meshHeading
pubmed-meshheading:9724033-Amino Acid Sequence, pubmed-meshheading:9724033-Animals, pubmed-meshheading:9724033-Animals, Newborn, pubmed-meshheading:9724033-Base Sequence, pubmed-meshheading:9724033-Cattle, pubmed-meshheading:9724033-DNA, Complementary, pubmed-meshheading:9724033-DNA-Binding Proteins, pubmed-meshheading:9724033-Embryo, Mammalian, pubmed-meshheading:9724033-Embryonic and Fetal Development, pubmed-meshheading:9724033-Female, pubmed-meshheading:9724033-Fushi Tarazu Transcription Factors, pubmed-meshheading:9724033-Gene Expression Regulation, Developmental, pubmed-meshheading:9724033-Gonads, pubmed-meshheading:9724033-Homeodomain Proteins, pubmed-meshheading:9724033-Male, pubmed-meshheading:9724033-Mice, pubmed-meshheading:9724033-Molecular Sequence Data, pubmed-meshheading:9724033-Receptors, Cytoplasmic and Nuclear, pubmed-meshheading:9724033-Sequence Homology, Amino Acid, pubmed-meshheading:9724033-Sex Differentiation, pubmed-meshheading:9724033-Steroidogenic Factor 1, pubmed-meshheading:9724033-Swine, pubmed-meshheading:9724033-Transcription Factors
pubmed:year
1998
pubmed:articleTitle
Porcine steroidogenic factor-1 gene (pSF-1) expression and analysis of embryonic pig gonads during sexual differentiation.
pubmed:affiliation
Centre de Recherche en Reproduction Animale, Department of Veterinary Biomedicine, Faculty of Veterinary Medicine, University of Montréal, St.-Hyacinthe, Québec, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't