Linked Life Data

9719625

Source:http://linkedlifedata.com/resource/pubmed/id/9719625

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1998-9-14
pubmed:abstractText
Single-strand confirmation polymorphism (SSCP) analysis on MDE gels has been extensively used to detect BRCA1 gene mutations. In screening a large cohort of ovarian cancer patients, our group was not completely satisfied with the standard published techniques. Although conventional primer sets usually amplified well, we were able to enhance band spread and resolution by varying the PCR and electrophoresis conditions for many of the individual exons. These alterations enhanced our ability to detect polymorphisms and mutations. When utilizing SSCP screening of a gene as large as BRCA1, no one set of conditions or even method may be optimal for all of the exons. Rather, a variety of different methods and conditions should be studied for each set of amplimers under analysis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1096-7192
pubmed:author
pubmed:copyrightInfo
Copyright 1998 Academic Press.
pubmed:issnType
Print
pubmed:volume
64
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
173-6
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Optimization of PCR and electrophoresis conditions enhances mutation analysis of the BRCA1 gene.
pubmed:affiliation
Department of Obstetrics and Gynecology, University of Iowa Hospitals and Clinics, Iowa City, Iowa 52242, USA.
pubmed:publicationType
Journal Article