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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1999-5-19
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pubmed:abstractText |
Immunocytological localization of omega-agatoxin IVA (omega-aga IVA)-sensitive Ca2+ channels involved in glutamate release from growth cones of cultured rat dorsal root ganglion (DRG) neurons was studied with field emission scanning electron microscopy (FE-SEM) and transmission electron microscopy (TEM). The omega-aga IVA-sensitive Ca2+ channels were visualized by labeling with immuno-gold particles (30 nm). FE-SEM and TEM images showed that immuno-gold particles were present in the area of growth cones as well as somata, and generally absent on neurite stem and fibroblasts. TEM images of vertical ultra-thin sections showed that the immuno-gold particles were present on the surface of the plasma membrane. Since the gold particles indicate the immunological presence of omega-aga IVA-sensitive Ca2+ channels, the Ca2+ channels involved in transmitter release are present on growth cones before making synapse formation.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0304-3940
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
24
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pubmed:volume |
251
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
93-6
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9718982-Animals,
pubmed-meshheading:9718982-Calcium Channels,
pubmed-meshheading:9718982-Cells, Cultured,
pubmed-meshheading:9718982-Ganglia, Spinal,
pubmed-meshheading:9718982-Microscopy, Electron, Scanning Transmission,
pubmed-meshheading:9718982-Neurons,
pubmed-meshheading:9718982-Neurotransmitter Agents,
pubmed-meshheading:9718982-Rats,
pubmed-meshheading:9718982-Rats, Wistar
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pubmed:year |
1998
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pubmed:articleTitle |
Visualization of calcium channels involved in transmitter release from neuronal growth cones.
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pubmed:affiliation |
Department of Anatomy, Nihon University School of Dentistry at Matsudo, Chiba, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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