Linked Life Data

9718713

Source:http://linkedlifedata.com/resource/pubmed/id/9718713

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1998-9-25
pubmed:abstractText
A rapid and accurate method to quantify zearalenone in corn is described. The method uses immunoaffinity chromatography for purification and high-performance liquid chromatography (HPLC) for detection and quantification of the toxin. Corn samples were extracted with acetonitrile-water (90:10, v/v) and the extract was diluted with water (1:10, v/v) and applied to a Vicam ZearalaTest immunoaffinity column. The column was washed with water and zearalenone was eluted with methanol and quantified by reversed-phase HPLC with fluorometric detection (lambda ex = 274 nm, lambda em = 440 nm) using acetonitrile-water-methanol (46:46:8, v/v) as mobile phase. Zearalenone recoveries from the ZearalaTest column were higher than 95%, and the column can hold a maximum of 4.0 micrograms of toxin. Average recoveries of zearalenone from corn spiked at levels of 0.1-10 micrograms/g ranged from 9 to 99.5%, with relative standard deviations of < 6%. The detection limit was 3 ng/g based on a signal-to-noise ratio of 3:1. Comparative analysis of 14 naturally contaminated samples using this method and the AOAC official method 985.18 showed a reasonable correlation (r = 0.87). Advantages of the immunoaffinity method as compared to the AOAC method are discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0021-9673
pubmed:author
pubmed:issnType
Print
pubmed:day
31
pubmed:volume
815
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
133-40
pubmed:dateRevised
2009-1-15
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Determination of zearalenone in corn by means of immunoaffinity clean-up and high-performance liquid chromatography with fluorescence detection.
pubmed:affiliation
Istituto Tossine e Micotossine da Parassiti Vegetali, C.N.R., Bari, Italy.
pubmed:publicationType
Journal Article