Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1998-9-10
|
| pubmed:abstractText |
The Notch signaling system regulates proliferation and differentiation in many tissues. Notch is a transmembrane receptor activated by ligands expressed on adjacent cells. Hematopoietic stem cells and early progenitors express Notch, making the stromal cells which form cell-cell contacts with progenitor cells candidate ligand-presenting cells in the hematopoietic microenvironment. Therefore, we examined primary stromal cell cultures for expression of Notch ligands. Using reverse transcription-polymerase chain reaction, in situ hybridization, immunohistochemistry, and Western blotting, we demonstrate expression of Jagged 1 in primary stromal cultures. To investigate if the stromal expression of Jagged 1 has functional effects on hematopoietic progenitors, we cultured CD34(+), c-kit+ hematopoietic progenitor cells derived from the aorto gonadal mesonephros region of day 11 mouse embryos on the Jagged 1(-) stromal cell line S17 and on S17 cells engineered to express Jagged 1. The presence of Jagged 1 increased the number of colonies formed in subsequent methylcellulose culture fourfold. Larger increases in colony numbers were observed under the same culture conditions with CD34(+), c-kit+ hematopoietic progenitor cells derived from d11 fetal liver. These results obtained in vitro table Jagged 1 as a candidate regulator of stem cell fate in the context of stromal microenvironments in vivo.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD34,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-kit,
http://linkedlifedata.com/resource/pubmed/chemical/RNA-Directed DNA Polymerase,
http://linkedlifedata.com/resource/pubmed/chemical/Serrate proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0006-4971
|
| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 1998 by The American Society of Hematology.
|
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
92
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1505-11
|
| pubmed:dateRevised |
2009-11-19
|
| pubmed:meshHeading |
pubmed-meshheading:9716576-Animals,
pubmed-meshheading:9716576-Antigens, CD34,
pubmed-meshheading:9716576-Aorta,
pubmed-meshheading:9716576-Blotting, Western,
pubmed-meshheading:9716576-Calcium-Binding Proteins,
pubmed-meshheading:9716576-Cells, Cultured,
pubmed-meshheading:9716576-Embryo, Mammalian,
pubmed-meshheading:9716576-Gene Expression,
pubmed-meshheading:9716576-Gonads,
pubmed-meshheading:9716576-Hematopoietic Stem Cells,
pubmed-meshheading:9716576-Immunohistochemistry,
pubmed-meshheading:9716576-In Situ Hybridization,
pubmed-meshheading:9716576-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:9716576-Membrane Proteins,
pubmed-meshheading:9716576-Mesonephros,
pubmed-meshheading:9716576-Mice,
pubmed-meshheading:9716576-Mice, Inbred C57BL,
pubmed-meshheading:9716576-Mice, Inbred CBA,
pubmed-meshheading:9716576-Polymerase Chain Reaction,
pubmed-meshheading:9716576-Proto-Oncogene Proteins c-kit,
pubmed-meshheading:9716576-RNA-Directed DNA Polymerase,
pubmed-meshheading:9716576-Stromal Cells
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
Stromal expression of Jagged 1 promotes colony formation by fetal hematopoietic progenitor cells.
|
| pubmed:affiliation |
Section of Gene Function and Regulation & Leukaemia Research Fund Centre, Chester Beatty Laboratories, Institute of Cancer Research, London, UK.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|