Linked Life Data

9715426

Source:http://linkedlifedata.com/resource/pubmed/id/9715426

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1998-11-4
pubmed:abstractText
We have previously reported on laser spectroscopy as a simple alternative to mass spectrometry. To validate a simplified 13C-urea breath test (UBT) with laser spectroscopy for the detection of Helicobacter pylori in clinical use, we evaluated the optimal time of breath sample collection. The 13C-UBT was carried out on each of 102 infected and 70 non-infected subjects (32 without eradication and 38 after eradication therapy). Breath samples were taken at five time points within 60 min followed by 100 mg of 13C-urea administration. The ratio of 13CO2 to 12CO2 was measured using laser spectroscopy and the recovery of tracer in the exhaled breath was calculated. Results were compared with histological and culture examinations of gastric biopsies to establish the infection status. For statistical evaluation of 13C-UBT, the optimal timing of breath sample collection was examined on the basis of the kinetics of delta-13CO2. In 32 H. pylori-negative patients (without therapy), the mean +/- 2SD of delta-13CO2 was at its minimum 20 min after urea ingestion whereas in H. pylori-positive patients, the mean +/- SD delta-13CO2 was maximum at 20 min. In addition, receiver operating characteristic (ROC) curve analysis showed that the cut-off value was estimated between 2.5-3.0 per mil (%0) at 20 min before therapy. Based on the histology and culture results, the sensitivity, specificity and positive and negative predictive values were 98.0%, 100%, and 94.1%, respectively. In conclusion, 13C-UBT with laser spectroscopy is a non-invasive, simple, sensitive and specific test to determine H. pylori status. Our findings suggest that in clinical use, measurements made at 20 min after substrate administration could be recommended for most sensitive and specific 13C-UBT results.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0815-9319
pubmed:author
pubmed:issnType
Print
pubmed:volume
13
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
732-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:9715426-2-Pyridinylmethylsulfinylbenzimidazoles, pubmed-meshheading:9715426-Adult, pubmed-meshheading:9715426-Amoxicillin, pubmed-meshheading:9715426-Anti-Bacterial Agents, pubmed-meshheading:9715426-Breath Tests, pubmed-meshheading:9715426-Clarithromycin, pubmed-meshheading:9715426-Drug Therapy, Combination, pubmed-meshheading:9715426-Female, pubmed-meshheading:9715426-Helicobacter Infections, pubmed-meshheading:9715426-Helicobacter pylori, pubmed-meshheading:9715426-Humans, pubmed-meshheading:9715426-Male, pubmed-meshheading:9715426-Middle Aged, pubmed-meshheading:9715426-Omeprazole, pubmed-meshheading:9715426-Penicillins, pubmed-meshheading:9715426-Predictive Value of Tests, pubmed-meshheading:9715426-Proton Pumps, pubmed-meshheading:9715426-Sensitivity and Specificity, pubmed-meshheading:9715426-Spectrum Analysis, pubmed-meshheading:9715426-Urea
pubmed:year
1998
pubmed:articleTitle
Analysis of the 13C-urea breath test for detection of Helicobacter pylori infection based on the kinetics of delta-13CO2 using laser spectroscopy.
pubmed:affiliation
Third Department of Internal Medicine, Kyoto Prefectural University of Medicine, Japan. ttana@basic.kpu-m.ac.jp
pubmed:publicationType
Journal Article