Linked Life Data

9714891

Source:http://linkedlifedata.com/resource/pubmed/id/9714891

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1998-10-29
pubmed:abstractText
Green fluorescent protein (GFP) is a valuable marker for intracellular protein localization. However the fusion of GFP with structural proteins can alter their properties, resulting in a loss of fusion protein localization, decreased GFP fluorescence or both. We describe a novel targeting approach based on noncovalent heterodimerization of GFP and cytoplasmic structural proteins. The formation of structural protein/GFP complexes was mediated by modified leucine zipper protein spacers designed to form high-affinity heterodimers. The complexes localized accurately to specific sites within cells, providing selective fluorescence labeling of subcellular structures such as microfilaments or focal contacts.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0736-6205
pubmed:author
pubmed:issnType
Print
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
298-302, 304
pubmed:dateRevised
2006-4-21
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Green fluorescent protein labeling of cytoskeletal structures--novel targeting approach based on leucine zippers.
pubmed:affiliation
National Institute of Dental Research, NIH, Bethesda, MD, USA.
pubmed:publicationType
Journal Article