Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1998-10-29
pubmed:abstractText
A technique is described that allows the concurrent synthesis of homologous regions of separate oligonucleotides. The technique utilizes synthesis columns that are readily interconverted between single-chambered and dual-chambered. The regions of the oligonucleotides that differ are synthesized separately with single-chamber columns, and with the single molecules joined, the homologous regions synthesized simultaneously. After synthesis, the chambers are opened separately, the solid-phase-bound oligonucleotides are placed in deprotection vials and treated as normal. Compared with standard syntheses, no decrease in yield or oligonucleotide quality was observed. This technique can result in significant savings in time and reagent costs when synthesizing a series of homologous oligonucleotides. This technique could be extended to the simultaneous synthesis of more than two oligonucleotides, possibly up to four or five oligonucleotides in an appropriate multichambered column.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0736-6205
pubmed:author
pubmed:issnType
Print
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
234-8
pubmed:dateRevised
2000-12-18
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Simultaneous synthesis of partially homologous oligonucleotide sequences.
pubmed:affiliation
CSIRO, Division of Biomolecular Engineering, NSW, Australia.
pubmed:publicationType
Technical Report