Linked Life Data

9714534

Source:http://linkedlifedata.com/resource/pubmed/id/9714534

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1998-9-14
pubmed:abstractText
While extralenticular expression of proteins in the alpha crystallin (small heat shock protein) family is well documented, that for proteins in the beta/gamma-superfamily is less well established. Here we show, using SDS-PAGE, Western blotting and confocal microscopy, that there is a constitutive level of beta crystallin expression in mouse N1E-115 neural cells. Furthermore, upon heat shock at 43 degrees C or 55 degrees C, or cold shock at 30 degrees C, beta crystallin immunoreactivity translocated predominantly from the nuclear region into the cytoplasmic region of the cells. In conditions of stress, it may be important for beta crystallin to be recruited into the cytoplasm to stabilise other proteins via its high beta-sheet content, and/or to ensure that storage levels of cytoplasmic Ca2+ are maintained.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0014-5793
pubmed:author
pubmed:issnType
Print
pubmed:day
24
pubmed:volume
431
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
319-21
pubmed:dateRevised
2009-9-29
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Translocation of beta crystallin in neural cells in response to stress.
pubmed:affiliation
Division of Cell and Molecular Biology, School of Animal and Microbial Sciences, The University of Reading, Berkshire, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't