Linked Life Data

9713894

Source:http://linkedlifedata.com/resource/pubmed/id/9713894

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1998-9-14
pubmed:abstractText
This paper aims to evaluate different formats of the enzyme-linked immunosorbent assays (ELISAs) for detection of virus-specific antibodies and focuses on factors that may influence the diagnostic reliability of such tests. Newly developed and well-established ELISAs for detection of infections of bovine herpesvirus 1 (BHV1), bovine respiratory syncytial virus (BRSV), classical swine fever virus (CSFV), pseudorabies virus (PRV) and bovine viral diarrhoea virus (BVDV) are used as examples. Differences between competitive and non-competitive ELISAs are described, with special reference to the influence of the antigen, the conjugated antibody and the test sample on the test results. Attention is drawn to interference, which may result in false positive or false negative test results, with special emphasis on the 'bridging' phenomenon. The use of monoclonal antibodies and discriminatory tests are briefly discussed. Diagnostic reliability is described for tests that are used in monitoring or eradication programmes, emphasising the consequences of false negative and false positive test results. Finally, reducing assay-time and functional quality control for such tests are discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0253-1933
pubmed:author
pubmed:issnType
Print
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
550-61
pubmed:dateRevised
2005-11-16
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Critical factors affecting the diagnostic reliability of enzyme-linked immunosorbent assay formats.
pubmed:affiliation
DLO-Institute for Animal Science and Health, Department of Mammalian Virology, Lelystad, The Netherlands.
pubmed:publicationType
Journal Article, Review