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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1998-8-27
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pubmed:abstractText |
Expression of IL-12Rs is one important checkpoint for Th1 development. BALB/c DO11.10 CD4+ T cells stimulated by Ag in neutral conditions lose expression of the IL-12R beta 2 subunit and become unresponsive to IL-12. In contrast, B10.D2 or F1 (BALB/c x B10.D2) DO11.10 CD4+ T cells maintain IL-12R beta 2 expression when stimulated similarly. Here we show that the loss of IL-12 responsiveness by BALB/c T cells involves the action of endogenous TGF-beta. BALB/c T cells stimulated in the presence of anti-TGF-beta specifically maintain IL-12 responsiveness, express IL-12R beta 2 mRNA, and can stimulate nitric oxide production in peritoneal exudate cells. Low concentrations of TGF-beta added exogenously during primary activation of B10.D2 or F1 T cells significantly inhibit their development of IL-12 responsiveness. These effects of anti-TGF-beta are dependent on endogenous IFN-gamma and are inhibited by exogenously added IL-4. Thus, at least one effect of TGF-beta on Th1/Th2 development may be the attenuation of IL-12R beta 2 expression.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
161
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1664-70
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:9712029-Adjuvants, Immunologic,
pubmed-meshheading:9712029-Animals,
pubmed-meshheading:9712029-Antibodies, Monoclonal,
pubmed-meshheading:9712029-Cell Line,
pubmed-meshheading:9712029-Cells, Cultured,
pubmed-meshheading:9712029-Dose-Response Relationship, Immunologic,
pubmed-meshheading:9712029-Interleukin-12,
pubmed-meshheading:9712029-Lymph Nodes,
pubmed-meshheading:9712029-Lymphocyte Activation,
pubmed-meshheading:9712029-Mice,
pubmed-meshheading:9712029-Mice, Inbred BALB C,
pubmed-meshheading:9712029-Mice, Transgenic,
pubmed-meshheading:9712029-Receptors, Interleukin,
pubmed-meshheading:9712029-Receptors, Interleukin-12,
pubmed-meshheading:9712029-T-Lymphocytes, Helper-Inducer,
pubmed-meshheading:9712029-Transforming Growth Factor beta
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pubmed:year |
1998
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pubmed:articleTitle |
Low dose TGF-beta attenuates IL-12 responsiveness in murine Th cells.
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pubmed:affiliation |
Dartmouth Medical School-DHMC, Department of Pathology, Lebanon, NH 03756, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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