Linked Life Data

9712020

Source:http://linkedlifedata.com/resource/pubmed/id/9712020

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pubmed-article:9712020pubmed:abstractTextWe are interested in understanding the molecular basis of macrophage (Mphi) differentiation and activation by cytokines. Recent reports have suggested that the transcription factor STAT5 may play a role in Mphi differentiation. In the experiments described here, we assessed the expression of STAT5-related molecules in three Mphi cell lines, RAW 264.7, WEHI-3, and WEHI-3D+, which represent different stages of Mphi maturation, and also in primary peritoneal and bone marrow Mphi from BALB/c mice. The studies revealed that the previously characterized STAT5a and STAT5b isoforms are detectable at both the mRNA and protein levels in these Mphi populations. Additional STAT5-related proteins were detected by immunoblot analysis and were preferentially expressed in both the immature WEHI-3 cell population and the adherent bone marrow population containing immature Mphi. These results identify new isoforms of STAT5 and demonstrate that distinct patterns of expression of STAT5-related proteins are observed in Mphi at different stages of maturation.lld:pubmed
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pubmed-article:9712020pubmed:pagination1594-7lld:pubmed
pubmed-article:9712020pubmed:dateRevised2007-11-14lld:pubmed
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pubmed-article:9712020pubmed:articleTitleIdentification of an additional isoform of STAT5 expressed in immature macrophages.lld:pubmed
pubmed-article:9712020pubmed:affiliationDepartment of Medical Microbiology and Immunology, University of Wisconsin Medical School, Madison 53706-1532, USA.lld:pubmed
pubmed-article:9712020pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:9712020pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
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