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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1998-8-27
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pubmed:abstractText |
We are interested in understanding the molecular basis of macrophage (Mphi) differentiation and activation by cytokines. Recent reports have suggested that the transcription factor STAT5 may play a role in Mphi differentiation. In the experiments described here, we assessed the expression of STAT5-related molecules in three Mphi cell lines, RAW 264.7, WEHI-3, and WEHI-3D+, which represent different stages of Mphi maturation, and also in primary peritoneal and bone marrow Mphi from BALB/c mice. The studies revealed that the previously characterized STAT5a and STAT5b isoforms are detectable at both the mRNA and protein levels in these Mphi populations. Additional STAT5-related proteins were detected by immunoblot analysis and were preferentially expressed in both the immature WEHI-3 cell population and the adherent bone marrow population containing immature Mphi. These results identify new isoforms of STAT5 and demonstrate that distinct patterns of expression of STAT5-related proteins are observed in Mphi at different stages of maturation.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
161
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1594-7
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:9712020-Animals,
pubmed-meshheading:9712020-Bone Marrow Cells,
pubmed-meshheading:9712020-Cell Adhesion,
pubmed-meshheading:9712020-Cell Differentiation,
pubmed-meshheading:9712020-Cell Line,
pubmed-meshheading:9712020-DNA-Binding Proteins,
pubmed-meshheading:9712020-Immunoblotting,
pubmed-meshheading:9712020-Isomerism,
pubmed-meshheading:9712020-Macrophages,
pubmed-meshheading:9712020-Macrophages, Peritoneal,
pubmed-meshheading:9712020-Mice,
pubmed-meshheading:9712020-Mice, Inbred BALB C,
pubmed-meshheading:9712020-Milk Proteins,
pubmed-meshheading:9712020-Molecular Weight,
pubmed-meshheading:9712020-RNA, Messenger,
pubmed-meshheading:9712020-STAT5 Transcription Factor,
pubmed-meshheading:9712020-Trans-Activators
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pubmed:year |
1998
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pubmed:articleTitle |
Identification of an additional isoform of STAT5 expressed in immature macrophages.
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pubmed:affiliation |
Department of Medical Microbiology and Immunology, University of Wisconsin Medical School, Madison 53706-1532, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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